| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,118 | 0 | C | A | 56.0% | 7.7 / 37.0 | 25 | A422A (GCC→GCA) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (9/2); new base A (0/14); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTA > NZ_CP009273/3214032‑3214191 | gAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCaaa > 1:754897/1‑90 (MQ=255) tgttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGAt > 1:1010679/1‑90 (MQ=255) aaCGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCAtt > 1:434691/1‑90 (MQ=255) tttCCCAGGGAGTTGTCCCCCCGGCGCGTCCGGGGGGCCGAAC‑CCCTAAATAACGCAAAAACGATCCGCATTGAACCGCcactgacactg < 1:362375/90‑1 (MQ=255) ttgcccAGGGAGATTTCCCGCCGGCGCGTGCGGGGGGCCGAAA‑CGCTAAAAACCCAAAAAACGACCCGAATTAACCCGCcactgacactg < 1:833412/86‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:627589/1‑90 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:59263/1‑90 (MQ=255) tGCCGGGGAGTTGTCCCGCCGGCGCGTACGGGGGGCCGAAA‑CGCTAAAAAACGCAAAAACGACCCGCATTAAACCGCCactgacactgac < 2:853609/90‑1 (MQ=255) tGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCactgacactgac > 2:178544/1‑90 (MQ=255) gcccAGGGAGTTTTCCCGCCGGCGCGTCCGGGGGGCCGAAC‑CCCTAAAAACCCCAAAAACGACCCGAATTAAACCGCCactgacactgac < 1:425530/88‑1 (MQ=255) cAGGGAGATTTCCCGCCAGCGCGTACTGGTGGCCGGAC‑CCCTAAAAACCGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCAt < 2:129208/90‑1 (MQ=255) cAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGACCCGCATTGAACCGCCACTGACACTGACCAt < 2:194151/90‑1 (MQ=255) aGCGAGATTTCCCGCCGGCGCGTCTTGGTGGCCGAAA‑CGCTAAAAAACCCAAAAACGACCCGAATTAAACCGCCACTGACACTGACCAtt < 2:677129/90‑1 (MQ=255) agttGTCCCGCCGGCGGGTGCGGGGGGCCGGAA‑CGCTCAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:376609/87‑1 (MQ=255) agttGTCCCGCCGGCGCGTCCGGGGGGCGGGAC‑CGCTAAAAAACCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 2:866456/87‑1 (MQ=255) agttGTCCCGCCGGCGCGTACGGGGGGCCGGAA‑CGCTAAAAAACGCAAAAACGACCCGAATTAAACCGCCACTGACACTGACCATTGAAc < 1:275508/87‑1 (MQ=255) agaTTTCCCGCCAGCGCGTCCTGGTGGCCGGAA‑CGCTAAAAAACCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 2:412071/90‑1 (MQ=255) agaTGTCCCCCCAGCGCGTCCGGGGGGCGGGAA‑CGCTAAAAAACGCAAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 2:444995/90‑1 (MQ=255) ttGTCCCGCCGGCGCGTA‑TGGGGGGCGGAACCGCTAAATACCGCAAAAACGATCCGCATTGACCCGCCACTGACACTGACCATTGAACAg < 2:542518/89‑1 (MQ=255) ccGCCAGCGCGTACGGGTGGCCGGAA‑CCCTAAAAAACGCAAAAACGACCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGa < 2:341470/90‑1 (MQ=255) aGCGCGTACGGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGTACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 2:349897/90‑1 (MQ=255) cgcgTCCTGGGGCCCGAAC‑CGCTAAAAAACCCAAAAACGATCCGCATTGACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg < 1:318071/90‑1 (MQ=255) ggCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcggc > 1:89033/1‑90 (MQ=255) ccGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGgcgc > 1:126341/1‑90 (MQ=255) gAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTa > 1:100450/1‑90 (MQ=255) | GAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTA > NZ_CP009273/3214032‑3214191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |