| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 933,398 | 0 | A | C | 64.3% | 6.7 / 12.2 | 14 | T191P (ACC→CCC) | lolA | outer membrane lipoprotein chaperone LolA |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/9); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAAT > NZ_CP009273/933313‑933482 | cgcgGTGGAGCAGGACGGTCAGCGCAGCAGTTATCAACTAAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTcccccc < 1:125431/90‑1 (MQ=255) ggTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgcc > 2:363056/1‑90 (MQ=255) gTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCccgccg > 2:81904/1‑90 (MQ=255) gACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTc < 1:133543/90‑1 (MQ=255) gACGATCAGCGCAGCAGTTATCAACTAAAACCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTc < 1:216237/90‑1 (MQ=255) gATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACg > 1:118081/1‑90 (MQ=255) gATCAGCGCAGAAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACg < 2:39761/90‑1 (MQ=255) cAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTa < 2:623849/90‑1 (MQ=255) ttATCAACTGAAATCCCAGCAAAAGGGGGTTGTGGATGCAGCAAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACg < 1:120964/90‑1 (MQ=255) gAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTagag > 1:194897/1‑90 (MQ=255) ccAGCAAAAGGGGGCTGTGGATGCGGCAAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCt < 1:611449/90‑1 (MQ=255) gCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgag < 2:118081/90‑1 (MQ=255) aaaaTGGGGCTGTGGATGCAGCGAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCtgagtg < 2:530864/90‑1 (MQ=255) gAAATTTCCCTTCCCCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGAttttt < 2:58034/90‑1 (MQ=255) tCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGataat > 1:772602/1‑87 (MQ=255) | CGCGGTGGAGCAGGACGATCAGCGCAGCAGTTATCAACTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCAACGTAAGTAGAGGCACCTGAGTGAGCAATCTGTCGCTCGATTTTTCGGATAAT > NZ_CP009273/933313‑933482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |