| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,202,802 | 0 | T | G | 53.3% | 8.6 / 17.4 | 15 | V145G (GTC→GGC) | ymfQ | YmfQ family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCACCTGCTCATCGGCCTGTACTGACGCGGTGAATGCGCCGGAATGGCGGTATTACTGGCAGGTCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGATCCCTGTGATTCCGCACTGCGTATCTGGGGTGACACCGTT > NZ_CP009273/1202738‑1202887 | ttCACCTGCTCATCGGCCTGTACTGACGCGGTGAATGCGCCGGAATGGCGGTATTACTGGCAGGGCAACATGCCAGCCCCCACCCAcccc > 2:721486/1‑90 (MQ=255)ttCACCTGCTCATCGGCCTGTACTGACGCGGTGAATGCGCCGGAATGGCGGTATTACTGGCAGGGCAACAAGCCCGCCCCCACCAAcccc > 2:791659/1‑90 (MQ=255) tCGGCCTGTACTGACGCGGTGAATGCGCCGGAATGGCGGTATTACTGGCAGGTCAACATGCCAGCCACCACCAACTCCACCTGGATGACa < 2:232097/90‑1 (MQ=255) cTGTACTGACGCGGTGAATGCGCCGGAATGGCGGTATTACTGGCAGGGGAAAAAGCCCGCCCCCCCCCACTCCCCCCCGGTGAAATGTgg > 1:421002/1‑90 (MQ=255) aCGCGGTGAATGCGCCGGAATGGCGGTATTACTGGCAGGGCAACATGCCAGCCACCCCCAACTCCCCCCGGGTGACATTTGGGGATCCCt > 2:177458/1‑90 (MQ=255) aCGCGGTGAATGCGCCGGAATGGCGGTATTACTGGCAGGGCAACATGCCAGCCACCACCAACTCCACCCGGGTGACAAGTGGGGATCCCt > 2:636274/1‑90 (MQ=255) ggTGAATGCGCCGGAATGGCGGTATTACTGGCAGGGCAACATGCCAGCCCCCACCAACTCCACCTTGATGACATGTTGGGGTCCCcgtgg > 2:805965/1‑89 (MQ=255) aTGCGCCGGAATGGCGGTATTACTGGCAGGGCAAAAAGCCCGCCACCCCCCACCCCCCCCGGGTGACAATGGGGGATCCCCGTGGtttcc > 1:810000/1‑87 (MQ=255) tGCGCCGGAATGGCGGTATTACTGGCAGGTCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGATCCCTGTGATTCCGc < 1:493003/90‑1 (MQ=255) tGCGCCGGAATGGCGGTATTACTGGCAGGTCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGATCCCTGTGATTCCGc < 2:421002/90‑1 (MQ=255) cgcCGGAATGGCGGTATTACTGGCAGGTCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGATCCCTGTGATTCCGCAc < 2:108861/90‑1 (MQ=255) ggCGGTATTACTGGCAGGTCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGATCCCTGTGATTCCGCACTGCGTATCt < 2:713732/90‑1 (MQ=255) cAGGTCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGATCCCTGTGATTCCGCACTGCGTATCTGGGGTGACACCGtt > 1:454817/1‑90 (MQ=255) cAGGGCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGATCCCTGTGATTCCGCACTGCGTATCTGGGGTGACACCGtt > 2:332395/1‑90 (MQ=255) ggTCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGa < 2:771509/48‑1 (MQ=255) | TTCACCTGCTCATCGGCCTGTACTGACGCGGTGAATGCGCCGGAATGGCGGTATTACTGGCAGGTCAACATGCCAGCCACCACCAACTCCACCTGGATGACATGTGGCGATCCCTGTGATTCCGCACTGCGTATCTGGGGTGACACCGTT > NZ_CP009273/1202738‑1202887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |