| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,379,045 | 0 | T | G | 53.3% | 10.5 / 16.5 | 15 | G224G (GGT→GGG) | ycjX | YcjX family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATA > NZ_CP009273/1378958‑1379118 | gTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGGgc > 1:318148/1‑90 (MQ=255) cGATTATCTCCCCCACTGTAAAGAGCAGGGGCTGCCCTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCcgc < 1:547702/90‑1 (MQ=255) accacTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGGGGGCCCGCGCTTCaaattt > 2:597800/1‑86 (MQ=255) accacTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGGGGGCCCCCGCTGCAAtttt > 2:568060/1‑88 (MQ=255) cTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGGGCGCCCGCGCTGCAATTCTTccc > 2:503471/1‑90 (MQ=255) ggggCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGGGCGCCCCCGCTGCGATTTTTTCCGCGGGCGGgtgt > 1:651153/1‑90 (MQ=255) ggggCTGCACTTTATTCAGCCTGGGCGCCTTGTCTTGCCGGGAGATATGGCAGGGGGGGCCCCGCCGCTATTCTTCCCGTGGCCGGgtgt > 1:491136/1‑90 (MQ=255) ggCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCg < 2:649083/90‑1 (MQ=255) ttCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCg < 2:46082/90‑1 (MQ=255) gCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGGGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTc > 2:673908/1‑90 (MQ=255) gCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGGGCGCCCGCCCTGCCATTTTTTCCGGGGGCGGGTGTCGATAACTGGGGCggggc > 1:207104/1‑86 (MQ=255) ccTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGGGTcc > 2:241817/1‑90 (MQ=255) gCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGa < 2:729884/90‑1 (MQ=255) cGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATa < 1:597800/90‑1 (MQ=255) cGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATa < 2:449936/90‑1 (MQ=255) | GTGGACCGATTATCTCCACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGAGATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGCGAGTCCAAACTGGCGCAGGCCGATA > NZ_CP009273/1378958‑1379118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |