| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,559,878 | 0 | T | G | 53.8% | 12.7 / 17.0 | 13 | N38T (AAC→ACC) | dosP | oxygen‑sensing cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACGCAAATCCCGCGGAATCAGCATATCAATGTTATTGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGTTGAAAAACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGC > NZ_CP009273/1559792‑1559963 | gACGCAAATCCCGCGGAATCAGCATATCAATGTTATTGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGGTGa > 1:169376/1‑90 (MQ=255) ccGCGGAATCAGCATATCAATGTTATTGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGTTGAAAAACATCAc < 2:658218/90‑1 (MQ=255) tatCAATGTTATTGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGTTGAAAAACATCACTTCATCATTTTCGt < 1:103350/90‑1 (MQ=255) aTGTTATTGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGTTGAAAAACATCACTTCATCATTTTCGttaatt < 2:214445/90‑1 (MQ=255) gTTATTGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGGTGAAAAAAATCACTTCATCATTTTCGttaattaa > 2:643680/1‑90 (MQ=255) ttATTGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGTTGAAAAACATCACTTCATCATTTTCGTTAATTAac < 2:704328/90‑1 (MQ=255) ttGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGGTGAAAAAAATCACTTCTTCATTTTTGTTTATTAAAAcc > 1:626685/1‑90 (MQ=255) ccAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGTTGAAAAACATCACTTCATCATTTTCGTTAATTAACACCGCa < 2:314161/90‑1 (MQ=255) cAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGGTGAAAAAAATCACTTCATCCTTTTTGTTAATTAACACCGccc > 1:73787/1‑88 (MQ=255) aaTGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGTTGAAAAACATCACTTCATCATTTTCGTTAATTAACACCGCAcc < 2:169376/90‑1 (MQ=255) tttGTATCCCCAGAGCTTCTCTGCGGCGGGGGTGAGAAAAATCACTTCATCCTTTTTTTTCATTAAAACCCCACCCACCATATTTTtctc > 1:33126/1‑90 (MQ=255) ttGTATCCCCAGAGCTTCTCTGCGGCGGGGGTGAAAAAAATACCTTCCTCATTTTTGTTTATTAACACCGCACCCATAATAGTTTTCTCa > 1:89934/1‑90 (MQ=255) ggggGTGAAAAACATCACTTCATCATTTTTGTTAATTAACACCGCACCCCTCATATTTTTCTCAAAGGCGGGGGAAAAAAAACCATccgc > 1:749698/1‑90 (MQ=255) | GACGCAAATCCCGCGGAATCAGCATATCAATGTTATTGCCAATGACTTCTTCACGTTTGTATCCCCAGAGCTTCTCTGCGGCGGGGTTGAAAAACATCACTTCATCATTTTCGTTAATTAACACCGCACCCATCATATTTTGCTCAAGGGCGGGGAAAAAAATGCCATCGGC > NZ_CP009273/1559792‑1559963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |