| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,356,152 | 0 | C | A | 53.8% | 9.4 / 20.9 | 13 | A321S (GCC→TCC) | yfaY | nicotinamide mononucleotide deamidase‑related protein YfaY |
| Reads supporting (aligned to +/- strand): ref base C (6/0); new base A (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCA > NZ_CP009273/2356067‑2356235 | cGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCaaaa > 2:105356/1‑90 (MQ=255) gAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGc > 2:181802/1‑90 (MQ=255) gCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCccgccg > 2:205306/1‑90 (MQ=255) ccGTCGGGCGTGGCTAGCGAAAAGTTGAGATGCTCGTTCTCAAACCCCAAAACAGCCAGTGCCAGCCCGGAAAAATGGTTGGCCCGCCGt < 2:495232/90‑1 (MQ=255) ggggCTAGCCAAAATTGGAGTTGCTCTTTCTCAAACCCAAAAACAGCCAGGGCCCGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATc < 1:181802/88‑1 (MQ=255) gcgcAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcg > 1:314338/1‑90 (MQ=255) gcgcAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcg > 1:53243/1‑90 (MQ=255) cgaaaaGTGGAGATGCTCTTTCTCGAACCCCAAAACAGCCAGTGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcgc < 2:805077/87‑1 (MQ=255) agaTGCTCGTTCTCAAACCCCAAACCAGCCAGTGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGcc < 2:799392/90‑1 (MQ=255) acacCCAGCCCCAGCCCGGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAcc < 1:24361/90‑1 (MQ=255) gTGCCAGCCCGGCAATATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTcac > 2:723761/1‑90 (MQ=255) accccGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGcc < 2:690875/88‑1 (MQ=255) gCCCGGAAAATTGTTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCa < 2:552468/90‑1 (MQ=255) | CGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCA > NZ_CP009273/2356067‑2356235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |