| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,387,034 | 0 | T | G | 53.8% | 11.7 / 13.9 | 13 | T862P (ACG→CCG) | yhdP | AsmA2 domain‑containing protein YhdP |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATC > NZ_CP009273/3386958‑3387112 | aGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGGGCGGTTTTCTTTCTgcc > 2:383063/1‑90 (MQ=255) gggCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGGGGGGTTTTCTTTCTtccc > 2:656766/1‑89 (MQ=255) ggCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTgccgc < 1:680559/90‑1 (MQ=255) gccagccaCTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGGGGGGTTTTCTTTCTtccgcc > 2:167195/1‑90 (MQ=255) cTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGGGCGTTTTTCTGTCTTCCCCCCcaaaaac > 2:495195/1‑86 (MQ=255) aTTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGGGCGGTTTTCTGTCTGCCCCCCCAATAACACAATCGAg > 2:798454/1‑90 (MQ=255) aTCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTc < 1:115578/90‑1 (MQ=255) tCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGtttt < 1:423596/55‑1 (MQ=255) tCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGtttt > 2:423596/1‑55 (MQ=255) gcATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGGTTTTCTGTCTGCCCCCCCAAAAGCACGATCGAGCGGCAGCtttt > 2:529267/1‑90 (MQ=255) ttCAACGCCACTTTGTTCCGGCAACGGCGGGGGCGGTTTTCTTTCTGCCGCCCCAAAAACACCATCGAGCGTCAGCTTTTTACCGAGCaa > 2:59311/1‑90 (MQ=255) cAACGCCACTTTGTTCCGGCAACGGCGGGAGCGGTTTTCTGTCTGCCGCCCCAAAAACACGAACGAGCGGCCGCTTTTTTCCCCGCAAcc > 2:251687/1‑90 (MQ=255) aaCGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCa < 1:33087/90‑1 (MQ=255) gCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCg < 1:752455/90‑1 (MQ=255) cggcggGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATc < 2:208515/90‑1 (MQ=255) | AGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATC > NZ_CP009273/3386958‑3387112 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |