Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,012,9310AC54.5% 2.2 / 11.1 11T138P (ACA→CCA) ssnAputative aminohydrolase SsnA
Reads supporting (aligned to +/- strand):  ref base A (0/5);  new base C (6/0);  total (6/5)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCT‑CCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAAC  >  NZ_CP009273/3012846‑3013002
                                                                                      |                                                                        
ttGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGTGTTCT‑Ccccacc                                                                      >  1:263452/1‑88 (MQ=255)
       cTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCT‑CCCCCTTTcgcggc                                                               >  2:70315/1‑88 (MQ=255)
          gAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCT‑CCACATTGCGCGACG‑Ca                                                           <  1:70315/90‑1 (MQ=255)
                                    ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGCCCT‑CCCCCTTTCGCGGCGCCTTTTTTTAAATTTGGCc‑gcgcggggt                                 >  1:302258/1‑86 (MQ=255)
                                    ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCCCT‑CCCCCTTGCGCGGCG‑CATTTTTTTAAATTGGCCTGcgcgcggt                                 >  1:275013/1‑88 (MQ=255)
                                        aTCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGGTCTCCCCCATTCCCGGCCC‑CCTTTTTAAAAATTGGCC‑GCGCGCGGTGGcc                             >  1:121364/1‑90 (MQ=255)
                                                      ctctCCGGCGTATATCGGCGGGTCGCTCT‑CCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAactac               <  1:156850/90‑1 (MQ=255)
                                                        ctcCGGCGTATATCGGCGGGTCGCTCT‑CCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTg             <  2:302258/90‑1 (MQ=255)
                                                             gCGTATATCGGCGGGGCGCCCT‑CCCCATTGCGCGGCG‑CATTTTTTAAAAATTGCCTGCGCCCGGTGACCCGTTTTTTAAAAACTGAccct        >  2:232892/1‑88 (MQ=255)
                                                                 atatCGGCGGGTCGCTCT‑CCCCATTGCGCGACG‑CATTTTTTAAAATTGGCCTGCGCGCGGTGACCCGTTTTTTAAATACTGACCGTaaaa    >  2:124097/1‑88 (MQ=255)
                                                                   atCGGCGGGTCGCTCT‑CCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTaacaac  <  1:124097/90‑1 (MQ=255)
                                                                   atCGGCGGGTCGCTCT‑CCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTaacaac  <  1:222324/90‑1 (MQ=255)
                                                                                      |                                                                        
TTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCT‑CCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTACTGACCGTAACAAC  >  NZ_CP009273/3012846‑3013002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑