| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,341,237 | 0 | T | G | 54.5% | 8.3 / 10.2 | 11 | V255G (GTG→GGG) | rapZ | RNase adapter RapZ |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base G (6/0); total (8/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCA > NZ_CP009273/3341162‑3341325 | tACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCCTTCGGTGGATATTGGAGAGc > 2:317970/1‑90 (MQ=255) gCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGGATATTTCAGAGCAAATGGc > 2:238920/1‑90 (MQ=255) ggAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGGTCGGGGGATATTGCAGAGCAACTGGCAGa > 2:12409/1‑90 (MQ=255) ttGACGGTCGCCATTGGTTGTACCGGCGGGGAGAACCCGTCCGGGTGTATTTCAGAGGAACAGGGAGAGCACTTCCGCGCGCGCggggaa > 1:310980/1‑86 (MQ=255) gTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTc < 2:310980/90‑1 (MQ=255) cGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGGGTATATTTCAGAGCAACTGGGAGGCTACTTCCCCTCGCGCGGGAAAAAAGTCCa > 1:45886/1‑90 (MQ=255) ttGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCAc < 2:14518/90‑1 (MQ=255) ggTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGc < 2:78633/90‑1 (MQ=255) ggcggGAAGCACCGTTCGGGGTATATTGCAGAGCAACTGGCAGACTACTTCCCCTCCCGCGGGAAAAACGTCCCGGCACGCCCTCGTACg > 1:85978/1‑90 (MQ=255) gcggGAAGCACCCGTCGGGGTATATTGCAGAGCAACTGGCAGACTACTTCCCCCCGCGCGGGAAAAAAGTCCAGTCACGCCCTCGTACGc > 1:285547/1‑90 (MQ=255) gTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTa > 2:15263/1‑90 (MQ=255) gtgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCa > 2:216936/1‑90 (MQ=255) | TACCTATGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCA > NZ_CP009273/3341162‑3341325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |