Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,806,6320CT57.1% ‑3.2 / 11.0 14S181L (TCG→TTG) coaBCbifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC
Reads supporting (aligned to +/- strand):  ref base C (1/5);  new base T (8/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGG  >  NZ_CP009273/3806553‑3806703
                                                                               |                                                                       
aGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGCGCATTTTTTGCCCCTCAAc                                                               >  2:113997/1‑90 (MQ=255)
     ggCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTGCCCGGCAACGACCt                                                          >  2:64850/1‑90 (MQ=255)
          gtggtgATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAAc                                                     <  1:240136/90‑1 (MQ=255)
             gtgATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATc                                                  <  1:67392/90‑1 (MQ=255)
               gATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTg                                                <  2:41133/90‑1 (MQ=255)
                      gTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGCGCCTTTTTCCCCCGTCAAAGACCCAAAAAATCTGTACATTa                                         >  2:243120/1‑90 (MQ=255)
                       tCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTTCCCCTCAACCACCCGAAAAATCTGAACATTAt                                        >  2:191620/1‑90 (MQ=255)
                           gggCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGAtt                                    <  2:271267/90‑1 (MQ=255)
                                      cGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTTTCCCGTCAACGACCTGAAAAATCTGAACATTATGATTACCGCCGGccc                         >  2:358253/1‑90 (MQ=255)
                                       gATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCg                        <  1:64850/90‑1 (MQ=255)
                                                    ttGTGGATATGGCGGTAGCGCATTTTTTGCCCGTCAACGGCCTGAAAAATCTGAACATTATGATTACCGCCGGCCCCACGCGGGAAACGc           >  2:322725/1‑90 (MQ=255)
                                                      gtgGATATGGCGGTAGCGCCTTTTTTGCCCCCCAACAACCCGAAAAATCTTAACAATATGATTACCCCCCGCCCGCCCCGGGAACCGccc         >  1:217600/1‑88 (MQ=255)
                                                           tatGGCGGTAGCGCCTTTTTTGCCCCTCAACGACCTGAAAAATCTGAACATTATGATTACCGCCCGCCCCACCCGGGGACCCCCCGGTcc    >  1:3308/1‑90 (MQ=255)
                                                             tGGCGGTAGCGCATTTTTTGCCCCTCAACAACCTGAAACATCTTAACATTATGATTACCGCCCGCCCCCCCCGTGAACCCCTCCATcccg  >  1:130087/1‑88 (MQ=255)
                                                                               |                                                                       
AGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCATTTTTCGCCCGTCAACGACCTGAAACATCTGAACATTATGATTACCGCCGGCCCGACGCGTGAACCGCTCGATCCGG  >  NZ_CP009273/3806553‑3806703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑