| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,527,910 | 0 | T | A | 54.5% | 5.3 / 13.4 | 11 | I225F (ATT→TTT) | nanM | N‑acetylneuraminate epimerase |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base A (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGC > NZ_CP009273/4527825‑4527992 | aGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCACCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGc > 1:231569/1‑90 (MQ=255) gCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGcc < 1:69952/90‑1 (MQ=255) ggCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTa > 1:63595/1‑90 (MQ=255) cGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAggg > 2:220338/1‑90 (MQ=255) cccTGTTTTGGCTCCCCCATA‑AAAAAGCCAGG‑TTTTATCCCCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc < 2:63595/89‑1 (MQ=255) tgTTTTGCCTCCCCATTAAAAAAGCCAGT‑TTTTACCCCTTTTTTT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc < 2:245718/89‑1 (MQ=255) tttGGCTCCCCAATA‑AAAAACCAAGT‑TTTTACCCCTTTTATC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTa < 2:231569/90‑1 (MQ=255) ggCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACt > 1:245718/1‑90 (MQ=255) ctccGCAATT‑AAAAAGCCAGT‑TTTTACCCCTTTTTTT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc < 2:137153/87‑1 (MQ=255) ctccccAAT‑AAAAAGCCCGGTTTTTATCCCTTTTATC‑CACACCCGCCGCCCCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc < 1:353727/86‑1 (MQ=255) cATT‑AAAAACCCCGG‑TTTTTACCCCTTTTTTCCCCACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc < 1:144959/90‑1 (MQ=255) | AGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGG‑TTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGC > NZ_CP009273/4527825‑4527992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |