| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,921,519 | 0 | A | T | 57.1% | 4.2 / 17.0 | 14 | intergenic (+488/‑69) | ppnN/sdaC | nucleotide 5'‑monophosphate nucleosidase PpnN/HAAAP family serine/threonine permease SdaC |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base T (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATAACTTGTGTGTATCTTTCCGCCCTCAAATTATTACGGCGGTAAATGATTAAGCCATCGCCGATAGACAGATTTCATTTTTACGGTCAGGCACCTTCCCGGGCTGAACTGGCTAAAAGCTGAATTATTTGCATTCCTCCAGGAGAAATAGATGGAAAC > NZ_CP009273/2921436‑2921595 | cATAACTTGTGTGTATCTTTCCGCCCTCAAATTATTACGGCGGTAAAAGATTAAAACATCCCCCATAGACAGATTTTTTTTTTTCGGTCa > 1:191386/1‑90 (MQ=255) aCTTGTGTGTATCTTTCCGCCCTCAAATTATTACGGCGGTAAAAAATTAAGCCATCGCCGATAAACAAATTTTTTTTTTTCTGTCAGGCa > 1:335593/1‑90 (MQ=255) cTTGTGTGTATCTTTCCGCCCTCAAATTATTACGGCGGTAAATGATTAAGCCATCGCCGATAGACAGATTTCATTTTTACGGTCAGGCAc < 2:130501/90‑1 (MQ=255) tgtgtgTATCTTTCCGCCCTCAAATTATTACGGCGGTAAATAATTAAGCCATCGCCCGTAGAAAGATTTTATTTTTTCGGGCAGGCACCt > 1:283755/1‑90 (MQ=255) tgtgtgTATCTTTCCGCCCTCAAATTATTACGGCGGTAAAAGATTAAACCATCCCCCATAGACAAAATTTATTTTTTCGGTCAGGGACCt > 1:182705/1‑90 (MQ=255) tgtgtATCTTTCCGCCCTCAAATTATTACGGCGGTAAAAGATTAAACCATCGCCCATAGACAGATTTTATTTTTTCGGTCAGGGACCTTc > 1:37185/1‑90 (MQ=255) gtgtATCTTTCCGCCCTCAAATTATTACGGCGGTAAAAGATTAAGCCATCGCCCATAGACAGATTTCATTTTTTCGGGCAGGGACCTTcc > 2:256525/1‑90 (MQ=255) aTCTTTCCGCCCTCAAATTATTACGGCGGTAAATGATTAAGCCATCGCCCATAGACAGAATTTTTTTTTTCGGTCAGGCACCTTCCCggg > 1:347084/1‑90 (MQ=255) aaaTTATTACGGCGGTAAATGATTAAGCCATCGCCGATAGACAGATTTCATTTTTACGGTCAGGCACCTTCCCGGGCTGAACTGGCTaaa < 1:256525/90‑1 (MQ=255) taCGGCGGTAAAAGATTAAACCATCCCCGATAGACAAATTTTATTTTTTCGGTCAGGCACCTTCCCCGGCTGAACTGGCTAAAAACTGaa > 1:341828/1‑90 (MQ=255) tCGCCGATAGACAGATTTCATTTTTACGGTCAGGCACCTTCCCGGGCTGAACTGGCTAAAAGCTGAATTATTTGCATTCCTCCa < 1:122413/84‑1 (MQ=255) tCGCCGATAGACAGATTTCATTTTTACGGTCAGGCACCTTCCCGGGCTGAACTGGCTAAAAGCTGAATTATTTGCATTCCTCCa > 2:122413/1‑84 (MQ=255) aGATTTCATTTTTACGGTCAGGCACCTTCCCGGGCTGAACTGGCTAAAAGCTGAATTATTTGCATTCCTCCAGGAGAAATAGATGGAAAc < 1:69336/90‑1 (MQ=255) aGATTTCATTTTTACGGTCAGGCACCTTCCCGGGCTGAACTGGCTAAAAGCTGAATTATTTGCATTCCTCCAGGAGAAATAGATGGAAAc < 2:341828/90‑1 (MQ=255) | CATAACTTGTGTGTATCTTTCCGCCCTCAAATTATTACGGCGGTAAATGATTAAGCCATCGCCGATAGACAGATTTCATTTTTACGGTCAGGCACCTTCCCGGGCTGAACTGGCTAAAAGCTGAATTATTTGCATTCCTCCAGGAGAAATAGATGGAAAC > NZ_CP009273/2921436‑2921595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |