Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,358,437	C→T	L480F (CTT→TTT)	puuC →	aldehyde dehydrogenase PuuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,358,437	0	C	T	87.5%	19.2 / ‑3.0	8	L480F (CTT→TTT)	puuC	aldehyde dehydrogenase PuuC
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (4/3);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

AACTACAACGACGGCGATATGACCGTGCCGTTTGGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCCTTGAAAAATTCACTGAACTGAAAACCATCTGGATAAGCCTGGAGGCCTGAAATGACCGAACATACCAGCAGTTACTACGCCGCCAGTGC  >  NZ_CP009273/1358356‑1358526                                                                                  |                                                                                          aaCTACAACGACGGCGATATGACCGTGCCGTTTGGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCTTTGaaaaa                                                                                   <  2:110776/90‑1 (MQ=255)    tACAACGACGGCGATATGACCGTGCCGTTTGGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCTTTGAAAAATTc                                                                                >  1:74630/1‑90 (MQ=255)             ggCGATATGACCGTGCCGTTTGGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCTTTGAAAAATTCactgaactg                                                                       <  2:74630/90‑1 (MQ=255)                            ccGTTTGGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCTTTGAAAAATTCACTGAACTGAAAACCATCTGGATa                                                        <  2:222262/90‑1 (MQ=255)                              gTTTGGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCTTTGAAAAATTCACTGAACTGAAAACCATCTGGATAAg                                                      >  1:197690/1‑90 (MQ=255)                                ttGGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAACCCCTGCATGCCTTTGAAAAATTCACTGAACTGAAAACCATCTGGATAAGcc                                                    >  2:167147/1‑90 (MQ=255)                                                          ggTCGCGACAAATCCCTGCATGCCTTTGAAAAATTCACTGAACTGAAAACCATCTGGATAAGCCTGGAGGCCTGAAATGACCGAACATAc                          >  2:25218/1‑90 (MQ=255)                                                               cgACAAATCCCTGCATGCCCTTGAAAAATTCACTGAACTGAAAACCATCTGGATAAGCCTGGAGGCCTGAAATGACCGAACATACcagca                     <  1:315210/90‑1 (MQ=255)                                                                                  cTTGAAAAATTCACTGAACTGAAAACCATCTGGATAAGCCTGGAGGCCTGAAATGACCGAACATACCAGCAGTTACTACGCCGCCAGTGc  <  2:137118/90‑1 (MQ=255)                                                                                  |                                                                                          AACTACAACGACGGCGATATGACCGTGCCGTTTGGCGGCTATAAGCAGAGCGGCAACGGTCGCGACAAATCCCTGCATGCCCTTGAAAAATTCACTGAACTGAAAACCATCTGGATAAGCCTGGAGGCCTGAAATGACCGAACATACCAGCAGTTACTACGCCGCCAGTGC  >  NZ_CP009273/1358356‑1358526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑