| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 371,042 | 0 | A | C | 50.0% | 25.7 / 12.7 | 30 | Q43P (CAG→CCG) | mhpT | 3‑(3‑hydroxy‑phenyl)propionate transporter |
| Reads supporting (aligned to +/- strand): ref base A (2/13); new base C (15/0); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAAT > NZ_CP009273/370959‑371129 | gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGGGTCGCCCCGGCttt > 2:250218/1‑90 (MQ=255)gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGGTCGCCCCGGGttt > 2:589012/1‑90 (MQ=255)gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGGTCACCCCGGcccg > 2:1372140/1‑87 (MQ=255)gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGGttt > 2:531901/1‑90 (MQ=255)gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCctt > 2:1247073/1‑90 (MQ=255) aTCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGCTCGCCCCCGCTTTcccc > 2:701328/1‑87 (MQ=255) tCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCTTTTGCAc > 2:1377580/1‑90 (MQ=255) cGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACt < 2:1037020/90‑1 (MQ=255) gggCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTc < 1:842056/90‑1 (MQ=255) gggCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCCTTCGCCCTc > 2:1046475/1‑90 (MQ=255) ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATaa < 1:636649/90‑1 (MQ=255) ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGCATCGCCCCGGCTTTTGCACTCGATaa > 2:623734/1‑90 (MQ=255) gTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCCCCCCGGCTTTTGCACCCGATAAAATTCAAATg > 2:55498/1‑90 (MQ=255) cGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCTTTTGCACTCGATAAAAAGCAAAAggg > 2:703014/1‑90 (MQ=255) ctctGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCt < 2:1418303/90‑1 (MQ=255) ctctGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCt < 1:969079/90‑1 (MQ=255) gAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATAttt < 2:981504/90‑1 (MQ=255) aaGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCTTTTGCCCTCGATAAAAAGCAAATGGGGTGGGTAtttt > 1:302190/1‑89 (MQ=255) gggCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAgc < 1:1046475/90‑1 (MQ=255) ttCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATAc < 1:500032/90‑1 (MQ=255) tCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCCGGCTTTTGCACTCGATAAAATACAAAAGGGCTGGGTATTTAGGGCCGGGATACt > 2:871643/1‑90 (MQ=255) tCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTTGCACTCGATAAAATGCAAATGGGGTGGGTATTTAGGGCCGGGATACt > 2:278557/1‑90 (MQ=255) cAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTc < 1:789110/90‑1 (MQ=255) cAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTc < 1:496184/90‑1 (MQ=255) ggcggcTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCgg < 1:1247073/90‑1 (MQ=255) cTGGCATTGCGGCGGGGGGGATCGCCCCGGCCTTTGCCCCCCATAAAAAGCAAATGGGGGGGGTATTTTGCGCCCGGAAACTCCGttttc > 1:350694/1‑88 (MQ=255) tGCGGCGGGGGGCATCGCCCAGGCTTTCGCACTCGATAAAAAGCAAATGGGGTGGGTATTTTGCGCCGGGATACTCGGGTTGCTAccccg > 1:914063/1‑88 (MQ=255) ggggggCATCGCCCCGGCTTTCGCACTCGATAAAAAGCAAATGGGGTGGGTATTTAGCGCCGGAATAATCGGTTTGCTACCCCGCGCgtt > 1:227462/1‑90 (MQ=255) cGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCgg < 1:703014/90‑1 (MQ=255) ccAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAAt < 2:917346/90‑1 (MQ=255) | GACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTTGGTTGGCGGAAT > NZ_CP009273/370959‑371129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |