| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,557,645 | 0 | T | G | 57.9% | 17.1 / 21.7 | 19 | L782L (CTA→CTC) | dosP | oxygen‑sensing cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (11/0); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCG > NZ_CP009273/1557564‑1557723 | cccGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCg < 2:1166777/90‑1 (MQ=255) cGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGGAGGGGGGGGGGAaaaaaa > 1:296648/1‑90 (MQ=255) gggCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAat < 1:1003413/90‑1 (MQ=255) gggCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAat < 2:1355994/90‑1 (MQ=255) gggCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAat < 1:1002303/90‑1 (MQ=255) agagGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATccc < 2:48384/90‑1 (MQ=255) tCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAAcgcg < 2:1072376/90‑1 (MQ=255) tCAGCGGTAACACGCTGCTCATCCAGCCTTGAATTTCTTCGGCGGGGAGGGGGGGGGGAAAAAAAAATCCCCGAATAAAGCGACAggggg > 1:180259/1‑89 (MQ=255) gCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCt < 1:153734/90‑1 (MQ=255) tgctCATCCAGCCTGGAATTTCTTCGGCGGGGAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCAtct > 2:472877/1‑90 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGGAGGGGGGGGGGAAAGAAAAATCCCCCAATAAACCGACAGTGGATCTTGCGTAGCAtctc > 1:1240927/1‑90 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGGAGGGGGGGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGGTCTTGCGTAGCAtctc > 1:283279/1‑90 (MQ=255) gctCATCCAGCCTGGAATTTCTTCGGCGGGGAGGGGGCGGGGAAAGAAATATCCCTGAATAACGCGACAGGGGGTCTTGCGTAGCAtctc > 1:67971/1‑90 (MQ=255) aGCCTGGAATTTCTTCGGCGGGTAGGGGGGGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGct > 2:1418090/1‑90 (MQ=255) aGCCTGGAATTTCTTCGGCGGGGAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGct > 2:267779/1‑90 (MQ=255) aGCCTGGAATTTCTTCGGCGGGGAGGGGGCCGGAAAAAAAAAATCCCCCAATAACCCGGCAATGGATCTTGCCTCGCATCCCCAATTGct > 2:1336154/1‑90 (MQ=255) ccTGGAATTTCTTCGGCGGGGAGGGGGCGGGGAAAAAAATATCCCCGAAAAACACGACAGGGGGTCTTGCGGAGCATCTCAAATTTctct > 1:1190536/1‑90 (MQ=255) ccTGGAATTTCTTCGGCGGGGAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGGGGATCTTGCGTAGCATCTCAAATTGctct > 1:247644/1‑90 (MQ=255) tcttcGGCGGGGAGGGGGCGGGAAAAAAAAAATCCCTGAATAACGCGACAGTGGGTCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCg > 1:133379/1‑90 (MQ=255) | CCCGCGAGTGCGGGCGAGAGGAATTTGTCAGATTTTCAGCGGTAACACGCTGCTCATCCAGCCTGGAATTTCTTCGGCGGGTAGGGGGCGGGAAAAGAAATATCCCTGAATAACGCGACAGTGGATCTTGCGTAGCATCTCAAATTGCTCTTTGGTTTCG > NZ_CP009273/1557564‑1557723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |