Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,738,4480AC52.9% 36.0 / 53.7 34V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
Reads supporting (aligned to +/- strand):  ref base A (15/1);  new base C (0/18);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 8.62e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-08
Rejected as consensus: Frequency below/above cutoff threshold.

GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC  >  NZ_CP009273/2738359‑2738533
                                                                                         |                                                                                     
gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa                                                                                       >  2:1257104/1‑90 (MQ=255)
                       tGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCcgcg                                                                >  2:1372525/1‑90 (MQ=255)
                            cGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCttt                                                           >  1:297699/1‑90 (MQ=255)
                                     ttCGCTGCCGCCCGTTTGATCAAGCTTGCCCCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATg                                                  <  1:1440481/90‑1 (MQ=255)
                                       cGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGaa                                                >  2:9974/1‑90 (MQ=255)
                                          tGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATgg                                             <  1:498402/90‑1 (MQ=255)
                                             cGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGt                                          <  1:52626/90‑1 (MQ=255)
                                                  gCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGc                                     >  2:1016952/1‑90 (MQ=255)
                                                   cTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                    <  2:790302/90‑1 (MQ=255)
                                                   cTTAATCACGTTTGCGCCCTTGTGGTGAAAGAAAAATCCCCTTTGCCCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCa                                    <  1:1257104/90‑1 (MQ=255)
                                                    ttGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCaa                                   <  2:1115683/90‑1 (MQ=255)
                                                     tGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAg                                  <  2:324628/90‑1 (MQ=255)
                                                      gATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAgg                                 <  1:20135/90‑1 (MQ=255)
                                                        tCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGt                               <  2:185950/90‑1 (MQ=255)
                                                           aGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGc                            >  2:127533/1‑90 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCTTTTGCCCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  2:1028295/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  2:154775/90‑1 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          >  1:976159/1‑90 (MQ=255)
                                                             cTTGCGTCCCTGTGGTGAAAGAAAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:560300/90‑1 (MQ=255)
                                                             cTTCCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  2:62968/90‑1 (MQ=255)
                                                              ttGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAcc                         <  2:1020095/90‑1 (MQ=255)
                                                                     ccTGTGGTGACAGATAAATCCCCTTTGCGCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTa                  <  1:1025574/90‑1 (MQ=255)
                                                                      cTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          <  1:674401/81‑1 (MQ=255)
                                                                      cTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc                          >  2:674401/1‑81 (MQ=255)
                                                                             gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc          >  1:1401580/1‑90 (MQ=255)
                                                                             gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc          >  1:398509/1‑90 (MQ=255)
                                                                               cAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCgg        >  1:182693/1‑90 (MQ=255)
                                                                               cAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCgg        >  2:753844/1‑90 (MQ=255)
                                                                                 gATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTc      >  2:1036459/1‑90 (MQ=255)
                                                                                  aTAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAAAGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTcc     >  1:320519/1‑90 (MQ=255)
                                                                                    aaaTCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgc   <  2:137361/90‑1 (MQ=255)
                                                                                    aaaTCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgc   >  1:449614/1‑90 (MQ=255)
                                                                                     aaTCCCTTTTGCCCCTCCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgcc  <  1:1003727/90‑1 (MQ=255)
                                                                                     aaTCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATgg                                             >  1:995656/1‑47 (MQ=255)
                                                                                     aaTCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATgg                                             <  2:995656/47‑1 (MQ=255)
                                                                                         |                                                                                     
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCC  >  NZ_CP009273/2738359‑2738533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: