| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,126,661 | 0 | T | G | 56.5% | 12.7 / 29.3 | 23 | H219P (CAC→CCC) | yghS | dTMP kinase |
| Reads supporting (aligned to +/- strand): ref base T (1/9); new base G (13/0); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.22e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGG > NZ_CP009273/3126573‑3126747 | cGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtg < 1:752570/90‑1 (MQ=255)cGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGgtg < 2:359584/90‑1 (MQ=255) atcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGCCCaac > 2:707068/1‑88 (MQ=255) tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 2:367746/90‑1 (MQ=255) tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 2:1317066/90‑1 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCCTCCCGGTCa > 1:1096443/1‑90 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGttga > 2:1264614/1‑88 (MQ=255) aTCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCa > 2:1078515/1‑90 (MQ=255) ccATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTGGACCCCCCAGGTCAAg > 1:264880/1‑90 (MQ=255) gATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGGCCATCCCCGTCCAGGGTCTTTtcg > 1:1343149/1‑90 (MQ=255) aTCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcgc < 1:1012962/90‑1 (MQ=255) aTCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcgc < 1:707068/90‑1 (MQ=255) aaaGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGa < 2:797372/90‑1 (MQ=255) gcgcGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTTGCCCCGCCGTATCAAAGAGCTTTTTGCCATTAAATg > 2:746401/1‑90 (MQ=255) gcggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTc < 1:1078515/90‑1 (MQ=255) gcggcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTTCGCCCTTGAATGTc > 2:779795/1‑90 (MQ=255) gcgcGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCAAGGATCTTTTCGCCCTTGAATGTCAGTTgcggcgga > 2:1443611/1‑88 (MQ=255) tAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGGAACGGc > 2:404416/1‑90 (MQ=255) cTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCAAtttttt < 2:1123069/90‑1 (MQ=255) ttGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcgc < 1:739879/53‑1 (MQ=255) ttGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTgcgc > 2:739879/1‑53 (MQ=255) ttGCAGAATTTCATCGGCGGGGGGTTGCCCATCCAGTTCAAGGGTCTTTTCGCCCTTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTc > 2:368460/1‑90 (MQ=255) tGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTTCAGGATCTTTTCGCCATTGAAAGTCAGTTGGGGGGTGACGGGGGtttttttt > 1:1129273/1‑88 (MQ=255) tGCAGAATTTCATCGGCGGGGGGGCGCCCCTCCCGTTCAAGGATCTTTTTGCCCTTTAATGTCAGTTGGGGCGGAACGGCGGgttttttc > 1:133383/1‑88 (MQ=255) gggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtgg < 1:746401/90‑1 (MQ=255) | CGTTAAATCATCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGG > NZ_CP009273/3126573‑3126747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |