| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 595,032 | 0 | T | G | 57.1% | ‑2.1 / 15.2 | 14 | V288G (GTG→GGG) | cusA | Cu(+)/Ag(+) efflux RND transporter permease subunit CusA |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAG > NZ_CP009273/594946‑595110 | gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggtggt < 2:374410/90‑1 (MQ=255) cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGgggggggggggg > 2:743853/1‑88 (MQ=255) gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGggggggggg > 2:657327/1‑87 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTTCGGTCCCGGCaaaaagcc > 1:323886/1‑87 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCGGCAAAAAAGcc > 2:46345/1‑90 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGcc < 1:358287/90‑1 (MQ=255) gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGT‑CCCACAAAAAAGccc > 1:785060/1‑90 (MQ=255) gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCCGCGGT‑CCGGCAAAAAAGccc > 2:172522/1‑90 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTTCTTCGAA‑CCCGCCAAAAACCCCGAGGAGTGATTGccgccgggg > 1:224550/1‑87 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGa < 1:657327/90‑1 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGa < 1:268298/90‑1 (MQ=255) gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGACCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGGTCGCCGCCGGGGAGGAc > 1:381598/1‑90 (MQ=255) cTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaaa < 1:150992/90‑1 (MQ=255) aaaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAAc < 1:437293/55‑1 (MQ=255) aaaCGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGAT‑CCGGCAAAAAc > 2:437293/1‑55 (MQ=255) gcgaaggcgaagTGGCGGGCGGGGGGGGGGTCCTGCGAT‑CCCGCAAAAAAGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAAATGGaaa > 1:213323/1‑90 (MQ=255) ggcgggcgGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagtag < 2:224550/90‑1 (MQ=255) | GCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGAT‑CCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAG > NZ_CP009273/594946‑595110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |