| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,571,380 | 0 | A | C | 62.5% | 6.3 / 15.9 | 16 | V166G (GTA→GGA) | yddB | TonB‑dependent receptor plug domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/10); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGA‑TCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGATAATA > NZ_CP009273/1571320‑1571465 | cGATACCCCAATTCCCCCTTGCTACCATCAGCGTTGAAGCGTTTGA‑TCTTTGCATAAATTCCCCCGCCATTGAAGCGACCAAATTCAAcc < 1:333635/90‑1 (MQ=255)cGATAACCCAATTTCCCCTTGCTATCATCAGCGTTGAAGCGTTTGA‑TCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAAcc < 2:745587/90‑1 (MQ=255) gATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGA‑TCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCg > 2:552329/1‑90 (MQ=255) aCCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGA‑TCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGcac > 1:71991/1‑90 (MQ=255) ttGCTATCATCAGCGTTGAAGCGTTTGA‑TCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATaa < 2:270681/90‑1 (MQ=255) ttGCTATCATCAGCGTTGAAGCGTTTGA‑TCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATaa > 1:36988/1‑90 (MQ=255) caGTGTTGAAGC‑TTTGATTCTTTGAATAAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAt < 1:391883/90‑1 (MQ=255) caGCGTTGAAGCGTTTGT‑TCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAt < 2:548220/90‑1 (MQ=255) caGCGTTAAGGCTTTTGA‑TCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAt < 2:707398/90‑1 (MQ=255) aGCGTTGAAGCGTTTGA‑TCTTTGCACAAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACAtt < 2:459544/90‑1 (MQ=255) gTTTGA‑TCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGc > 1:45490/1‑90 (MQ=255) gA‑TCTTTGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGAc < 2:221933/90‑1 (MQ=255) tttGCATCAATTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATc < 1:272932/89‑1 (MQ=255) tttGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATc > 2:272932/1‑89 (MQ=255) aaTTCCCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGataata < 1:207415/90‑1 (MQ=255) aaTTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGataata < 2:36988/90‑1 (MQ=255) | CGATAACCCAATTTCACCTTGCTATCATCAGCGTTGAAGCGTTTGA‑TCTTTGCATCAATTACCCCGCCATTGAAGCGACCAAATTCAACCGGCACAAAACTGTCATAAAGCGTCACATTGTCCAGTAAGCTGACATCAAGATAATA > NZ_CP009273/1571320‑1571465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |