| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 57.1% | 6.1 / 14.7 | 14 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (0/6); new base G (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTA > NZ_CP009273/2089484‑2089660 | gCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGtt > 2:652879/1‑90 (MQ=255) tCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAg < 1:439890/90‑1 (MQ=255) cAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGtc < 1:13216/90‑1 (MQ=255) aGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGtct > 1:687882/1‑90 (MQ=255) gacagTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGgcgc < 1:54169/86‑1 (MQ=255) ggCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATaa < 2:237577/90‑1 (MQ=255) ggAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAAt < 2:571771/90‑1 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTTAGTCTCTTGGAGAAGGCGCGGAAAATTTTTTCTTTTGCGCGGGGAAAAATTTCAt > 1:790957/1‑90 (MQ=255) gATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAATCTCTGGAAGATGCCGCGAAAATTTTTTTCTTTTGGGCGGGTAAAAatttgtt > 2:54169/1‑86 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAAATGCCGCGAAAATTTTTTCCTTTTGCGCGGGTAAAATTTTCATCAACtctc > 2:606814/1‑90 (MQ=255) ttCCGTTTTGTGTGGCGGGGGGGGTTAAAGTTCTGGGAGATGCCGCGAAAAATTTTTTCTTTTGGGCGGGGAAAAATTTTATCAACtctc > 1:700294/1‑90 (MQ=255) gtgtgGCGGGTGGGGTTTAATTTCTGGAAGATGCCGCGAAAATTTTTTTCTTTTGCGCGGATAAAAATTTCATCAACCCTCCTGCGCCgg > 1:689137/1‑90 (MQ=255) tgGCGGGGGGGGTTAAGGCTCTGGAAGATGCCGCGAAAAATCTTTTCTTTTGCGCGGGGAAAATTTTCCTCAAACCTCCTGCGCTGGCAg > 1:122944/1‑90 (MQ=255) gggTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAccc < 2:182522/90‑1 (MQ=255) aTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACatta > 2:746612/1‑90 (MQ=255) | GCTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTA > NZ_CP009273/2089484‑2089660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |