| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,832,718 | 0 | T | G | 57.1% | 12.0 / 14.9 | 14 | intergenic (‑95/‑165) | ascG/ascF | DNA‑binding transcriptional regulator AscG/PTS cellobiose/arbutin/salicin transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGT > NZ_CP009273/2832636‑2832794 | tttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaa < 1:425577/90‑1 (MQ=255) aGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTTAGGGGACCGGGTTCACAAATATAAAAAAAGAACAAt > 1:105620/1‑90 (MQ=255) aGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAAt > 2:805629/1‑90 (MQ=255) tgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTc > 1:594323/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAAAATTTAGGGGGCCGGGTTCACAAAAATAAAAAAAGAACAATTTActctc > 1:584691/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGGCCGGGTTCACAAATATAAAAAATGAACAATTTActctc > 1:296606/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATAATCAGGGGGCCCGTTACTCAAATAAAAAAAATGTAGAATTCActttt > 2:662528/1‑87 (MQ=255) ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTg > 2:388876/1‑90 (MQ=255) ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTTAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTTACTCTCTTg > 1:371293/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTCACAAATATAAAAAAAGAACAATTCACTCTCTTGCt > 2:507914/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTCAGGGGGCCGGGCTCGAAAATATAAAAAAAGAACAATTCACTCTCTTTCt > 1:398406/1‑90 (MQ=255) tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACa < 2:526618/90‑1 (MQ=255) ttCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt < 1:388876/90‑1 (MQ=255) ttCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt < 2:278/90‑1 (MQ=255) | TTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGT > NZ_CP009273/2832636‑2832794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |