| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,012,922 | 0 | T | G | 56.2% | 9.4 / 20.1 | 16 | S135A (TCG→GCG) | ssnA | putative aminohydrolase SsnA |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (9/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTAC > NZ_CP009273/3012837‑3012989 | cGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGct < 2:235744/90‑1 (MQ=255) ggACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGgcgcgc > 2:195807/1‑88 (MQ=255) ggACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCgggc > 1:630908/1‑87 (MQ=255) ggACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGctc > 1:378237/1‑90 (MQ=255) gACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGctct < 2:378237/90‑1 (MQ=255) gTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGctct < 1:231342/81‑1 (MQ=255) gTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGCCCTCcccactcc > 2:623180/1‑86 (MQ=255) gTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGctct > 2:231342/1‑81 (MQ=255) gATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGCTCTCCCCCCTTCGCGCCG‑CCttttt > 1:620305/1‑90 (MQ=255) gATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCCCCCTTCGCGGCG‑CCttttt > 1:155332/1‑90 (MQ=255) ggATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGGTCTCCCCCCTTCGCGGCGCCCTTTTTTAAAATTgg > 1:759861/1‑90 (MQ=255) ggATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGGGCCCTCCCCACTTCCCGGCGCCCTTTTTAAAATTTgc > 1:279626/1‑89 (MQ=255) cGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGa < 2:279626/90‑1 (MQ=255) ggTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGGCGCTCTCCACATTGCGCGGCGCCTTTTTTTAAATTTGGCc‑gcgcgcggt > 2:259861/1‑88 (MQ=255) aTCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGAcc < 1:93215/90‑1 (MQ=255) aTCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGAcc < 2:379755/90‑1 (MQ=255) ctctACGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAactac < 2:670609/90‑1 (MQ=255) | CGGACTGATTTGTTCCCTGGAAGCGATTAAGAGCGGATGTACATCGGTTATCGATCACCATGCCTCTCCGGCGTATATCGGCGGGTCGCTCTCCACATTGCGCGACG‑CATTTTTAAAAGTTGGCCTGCGCGCGATGACCTGTTTTGAAACTAC > NZ_CP009273/3012837‑3012989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |