| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,419,801 | 0 | T | G | 56.5% | 11.5 / 26.4 | 23 | L41V (TTG→GTG) | cycA | D‑serine/D‑alanine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base T (6/4); new base G (13/0); total (19/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.37e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTC > NZ_CP009273/4419712‑4419887 | atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt < 2:286376/90‑1 (MQ=255)atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt < 1:262927/90‑1 (MQ=255)atCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt < 1:653064/90‑1 (MQ=255) tCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGCCCCTTGGTCAGGGgtt > 1:37255/1‑90 (MQ=255) cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGTGGtttt > 2:338852/1‑89 (MQ=255) ccGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTg > 2:170023/1‑90 (MQ=255) cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTgg > 2:233450/1‑90 (MQ=255) cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCTTGGGACGGGGGGGTTTTTgg > 1:341955/1‑90 (MQ=255) cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCATTGGGACGGGGGTGTTTATgg > 2:653064/1‑90 (MQ=255) tACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCCTTTGGACGGGGGTGTTTTTGGGGGCTGGCAAAAAGa > 1:792432/1‑90 (MQ=255) cgcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGc < 1:741116/90‑1 (MQ=255) aTCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTGGGGGCTTGCAAAAAGATTAGCCTTg > 1:76720/1‑90 (MQ=255) aTCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGGCTGGCAAAACGATTAGCCTTg > 2:155745/1‑90 (MQ=255) acaAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCggg > 2:67041/1‑90 (MQ=255) cAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGGCTGGCAAAAAGATTAGCCTTTCCGGGGCGGCGGTCAttttt > 1:249681/1‑89 (MQ=255) gCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTTTTGGGGCTGGCCAAAAGATTAGCCTTGCCGGGCCGGCGGTCCTTTTTGt > 1:546977/1‑90 (MQ=255) tATTGCCATTGGCGGTGCCATTGGTACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTta > 1:280117/1‑90 (MQ=255) ttGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata > 2:267108/1‑90 (MQ=255) ttGCCATTGGCGGTGCCATTGGGACGGGGGTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTtata > 2:164469/1‑90 (MQ=255) aTTGGCGGTGCCATTGGTACGGGGGTGGTTTTGGGGTCTGGCAAAACGATTAGCCTTGCCGGGGCGGCGGTCCTTTTTGTTTATATGGTc > 1:643531/1‑90 (MQ=255) ggCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAtt < 2:365338/90‑1 (MQ=255) ggCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCAtt < 1:170023/90‑1 (MQ=255) tACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCtt > 2:261698/1‑90 (MQ=255) tACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCtt > 1:163785/1‑90 (MQ=255) tACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGACCATTGGTTTTATGCCCtt < 2:38955/90‑1 (MQ=255) ggggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCttttt > 2:503015/1‑90 (MQ=255) gggTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTc > 1:177182/1‑90 (MQ=255) | ATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTC > NZ_CP009273/4419712‑4419887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |