| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,725,079 | 0 | T | G | 58.3% | 7.4 / 11.4 | 12 | V579G (GTA→GGA) | lhr | ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGCGGATCCAGCAAATCACCCGCGATCAGGTGATTGTG > NZ_CP009273/1724993‑1725164 | ggATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAAATGGATGAGGGGATGGgata > 1:6888/1‑90 (MQ=255) gTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGGGAACTGGATGAGGAGATGGGATATGAgg > 1:83405/1‑89 (MQ=255) gTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGGTGAGGAGAGGGGATATGAGt > 1:445378/1‑90 (MQ=255) gTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGAGGGGATGGGATATGAgg > 1:205547/1‑89 (MQ=255) attaCCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGAGGAGAAGGGATATGAGGCGCGGGGGGAAGACATTAt > 2:15475/1‑90 (MQ=255) attaCCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAAATGGATGAGGAGATGGGATATGAGTCGCGGGGGGAAGACATTAt > 2:15474/1‑90 (MQ=255) aCCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGGTGAGGGGATGGGATATGAGTCGCGGGGGGAAGACATTATTAc > 2:249/1‑90 (MQ=255) ccGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGGGGGGGAACTGGATGAGGAGATGGGATATGAGTCGCGGGGGGACGACATTATCActc > 2:15680/1‑90 (MQ=255) tGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGgcgc < 2:314830/90‑1 (MQ=255) gCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATgg < 1:15680/90‑1 (MQ=255) ttCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGCGGAt < 1:218881/90‑1 (MQ=255) gcggcgGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGCGGATCCa < 1:291482/90‑1 (MQ=255) aTGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGCGGATCCAGCAAATCACCCGCGATCAGGTGATtgtg < 2:316592/90‑1 (MQ=255) | GGATCGTGGCATGTATAGCGTGTTATTACCCGAAGGTGAAGAAAAGGCCGGTTCGCGGCGGGTGGGTGAACTGGATGAGGAGATGGTATATGAGTCGCGGGTGAACGACATTATCACTCTCGGCGCTACCTCATGGCGGATCCAGCAAATCACCCGCGATCAGGTGATTGTG > NZ_CP009273/1724993‑1725164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |