| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,343,100 | 0 | A | C | 58.8% | 9.2 / 18.1 | 17 | pseudogene (36/105 nt) | BW25113_RS26180 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/10); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTAATGAAACGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGAT > NZ_CP009273/2343012‑2343167 | cTTAATGAAACGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTccac < 1:89898/90‑1 (MQ=255) tGAAACGTGTCGTATTAATGAATTTCTTTTGCCACTCCTACCTTGCCCCCCCTTTTCCCCTTCATTTGCAGCGCATAGATTCCCCCAGcc < 2:447384/90‑1 (MQ=255) tGAAACGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGCCCCCGCTTTTACCCTTCATTTGCAGCGCATAGATCCCCCCAGcc < 2:264944/90‑1 (MQ=255) aaaCGTGTCGTATTAATGAATTTCTTTTGCCACTCCTAGCTTGACCCCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCCCCAGccac < 2:195007/90‑1 (MQ=255) aaaCGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCCCCAGccac < 2:143903/90‑1 (MQ=255) aCGTGTGGTATTAATGATTTTCTTTTGCCACTACTAGCTTCCCGCCCCTTTTACCCTTCATTTGCAGCGCATAGATTCCCCCAGccacta < 2:475364/90‑1 (MQ=255) aCGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGccacta < 1:169301/90‑1 (MQ=255) gtgtCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACCCCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCCCCAGccactacc < 2:130901/90‑1 (MQ=255) gtgcCGTATTAATGAATTTCTTTTCCCCCTACAAGCTTGCCCCCGCTTTTCCCCTTCATTTGCAGCGCATAGATCCCCCCAGccactacc < 2:320694/90‑1 (MQ=255) aTTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATa > 1:26646/1‑90 (MQ=255) gACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAAt > 1:395583/1‑90 (MQ=255) gACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAAt > 1:381790/1‑90 (MQ=255) accccGCTTTTACCCTTCATTTGCAGCGAATAGATCCCCCCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATc < 2:381790/87‑1 (MQ=255) acacCGCTTTTCCCCTTCATTTGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATc < 1:207811/90‑1 (MQ=255) cacCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATc > 2:18974/1‑89 (MQ=255) cacCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATc < 1:18974/89‑1 (MQ=255) ttCATTTGCAGCGCATAGATTCCCCCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGa < 2:26646/90‑1 (MQ=255) tCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGAt > 2:123760/1‑90 (MQ=255) | CTTAATGAAACGTGTCGTATTAATGAATTTCTTTTGCCACTACTAGCTTGACACCGCTTTTACCCTTCATTTGCAGCGCATAGATTCCACCAGCCACTACCACTAATAGTGCGCCCAGCAGATAAAATGTGAATTTAATCAGGCGGATACAGCGAT > NZ_CP009273/2343012‑2343167 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |