| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,678,659 | 0 | A | C | 60.0% | 12.1 / 15.2 | 15 | Y70D (TAT→GAT) | glyA | serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/9); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGAGCCGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCC > NZ_CP009273/2678570‑2678732 | ggAGCCGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATa > 2:117071/1‑90 (MQ=255) cGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGc > 1:275572/1‑90 (MQ=255) cGTAGCCAGCGCCAAACATTTTTTCCGCACGATCGACCGCCATTTGTTAAACGATACCAACATCCCCGCAACCGCCGTAGTAGCGTTTGc < 1:277103/90‑1 (MQ=255) gTAGCCAGCGCAGACCAGTTCTTCCGCCCGATCGCCCCCCAGTTGTTAAACGATATAAACATCCCCGCAACCGCCGTAGTAGCGTTTGcc < 1:225132/90‑1 (MQ=255) gcgcCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATaa > 1:162090/1‑90 (MQ=255) aCAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAg < 1:44306/90‑1 (MQ=255) gTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGcct > 2:359422/1‑88 (MQ=255) ttCTTTCCCACGATCGATCGCCAGTTGTTCAACGATATCAACATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCata < 1:17937/90‑1 (MQ=255) gaatcgctcgCAATTTGTTCAACAATACCAACATCCCCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 1:63308/88‑1 (MQ=255) cgttcgatcgCTTGTTTTCCAACGATACAAACATCCCCCAACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 1:110245/87‑1 (MQ=255) cgagcgacCGCTTGTTTTTAAACAATACAAACATCCCCGCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTc < 2:151988/90‑1 (MQ=255) aGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAAccc > 2:340496/1‑90 (MQ=255) aGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAAccc > 2:93958/1‑90 (MQ=255) gttgttCAACGATATAAACATCCCCCCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCt < 2:99219/90‑1 (MQ=255) gttCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTgcg > 1:39990/1‑90 (MQ=255) taaaCGATACAAAAATCCTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGcc < 1:117071/88‑1 (MQ=255) | GGAGCCGGAGTGCGGCTGGACGTTAGCGTAGTCAGCGCCGAACAGTTCTTTCGCACGATCGATCGCCAGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCC > NZ_CP009273/2678570‑2678732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |