| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,090,826 | 0 | T | G | 60.0% | 6.4 / 12.2 | 15 | G67G (GGT→GGG) | hemW | radical SAM family heme chaperone HemW |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAA > NZ_CP009273/3090748‑3090910 | gCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGTGCGCCGAGccc > 2:270989/1‑89 (MQ=255)gCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGAGGCt > 1:212984/1‑90 (MQ=255) tGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGAGGCCGCttt > 2:25633/1‑90 (MQ=255) ttAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGAGCCCGCttttcc > 1:441738/1‑87 (MQ=255) cTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCg < 1:84219/90‑1 (MQ=255) cTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGCGCCTGCTTTcccgccccggg > 1:179339/1‑86 (MQ=255) cTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGGCGCCCAGGCTGCTTTTCCGCCCGGCg > 2:258858/1‑90 (MQ=255) cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAAc < 1:463490/90‑1 (MQ=255) gtgGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCCGCGATGCAAACgctg > 1:143513/1‑90 (MQ=255) tACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGAc < 1:127686/90‑1 (MQ=255) gTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCCGCTTTTCGGCCCCGCGGTGCAAACGCCGCTGGGCAGCGGGCGGgcgc > 2:151775/1‑90 (MQ=255) tAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGc < 2:68768/90‑1 (MQ=255) aaaGACAATCTTTATTGGCGGTGGGACGCCGAGCCCGCTTTTCGGCCCCGCGATGCAAACGCTGCTGGGCGGGGGGGGGGGGCGGTTGcc > 2:176377/1‑90 (MQ=255) aGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGc < 2:212984/90‑1 (MQ=255) gACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCGGCGGTGCAAAAGCTGCTGGGCGGGGGGCGTGCGCGGTTGCCGCt > 1:236916/1‑90 (MQ=255) ggtggtACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGaa < 1:349885/90‑1 (MQ=255) ggtggtACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGaa < 2:464476/90‑1 (MQ=255) | GCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAGAA > NZ_CP009273/3090748‑3090910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |