| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,118 | 0 | C | A | 58.8% | 4.3 / 24.4 | 17 | A422A (GCC→GCA) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (6/1); new base A (0/10); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGG > NZ_CP009273/3214044‑3214173 | aaCGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAtt < 1:136106/90‑1 (MQ=255) ggCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCca > 1:326036/1‑90 (MQ=255) tttGCCGGGGAGTTGTCCCGCCGGCGCTTACTGGTGGCCGGAACGCTAAATAACGCAAAAACGACCCGCATTGAACCGCcactgacactg < 1:40461/90‑1 (MQ=255) tttCCCAGGGAGATTTCCCGCCGGCGCGTTCGGGGGGCGGGACCGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCcactgacactg < 1:422767/90‑1 (MQ=255) ttccccAGGGAGTTTTCCCCCCGGCGCGTACGGGGGCCCGGACCCCTAAAAAACCCAAAAACGCCCCCCATTAAACCGCcactgacactg < 2:94226/86‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTATTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:55086/1‑90 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 1:19473/1‑90 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:422767/1‑90 (MQ=255) tGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCactgacactgac > 1:137092/1‑90 (MQ=255) gagtTGTCCCGCCGGCGGGTCCGGGGGGCCGGAACCCTCAAAACCCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGaa < 1:308146/90‑1 (MQ=255) agaTTTCCCGCCGGCGCGTAGGGGGGGCCGGAACGCAAAAAAACCCAAAACCGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc < 1:251020/90‑1 (MQ=255) agaTTTCCCCCCGGCGCGTACTGGGGGCCGGAACGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:22421/90‑1 (MQ=255) ttGTCCCGCCAGCGCGTTTGGGTGGCCGGAACGCTAAAAACCCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAg < 1:55086/89‑1 (MQ=255) ccgcccgcGGGTGGTGGTGGCGGGACCCCTAAAAAACGCAAAAACGACCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGa < 1:254439/90‑1 (MQ=255) gCCAGCGCGTACTGGTGGCCGGAACGCTAAATAACGCAAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAAc < 1:185448/90‑1 (MQ=255) cccAGCGCGTACGGGGGCCCGAAACCCAAAAAAACACAAAAACGACCCGAATAAAACCGCCACTGACACTGACCATTGAACAGTGTGAAc < 2:411300/89‑1 (MQ=255) aGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg > 2:422822/1‑90 (MQ=255) | AACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGG > NZ_CP009273/3214044‑3214173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |