| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,126 | 0 | T | C | 55.0% | 12.1 / 24.0 | 20 | I425T (ATC→ACC) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (7/2); new base C (0/11); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAG > NZ_CP009273/3214044‑3214216 | aaCGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCAtt < 1:136106/90‑1 (MQ=255) ggCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCca > 1:326036/1‑90 (MQ=255) tttGCCGGGGAGTTGTCCCGCCGGCGCTTACTGGTGGCCGGAACGCTAAATAACGCAAAAACGACCCGCATTGAACCGCcactgacactg < 1:40461/90‑1 (MQ=255) tttCCCAGGGAGATTTCCCGCCGGCGCGTTCGGGGGGCGGGACCGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCcactgacactg < 1:422767/90‑1 (MQ=255) ttccccAGGGAGTTTTCCCCCCGGCGCGTACGGGGGCCCGGACCCCTAAAAAACCCAAAAACGCCCCCCATTAAACCGCcactgacactg < 2:94226/86‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTATTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:55086/1‑90 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 1:19473/1‑90 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:422767/1‑90 (MQ=255) tGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCactgacactgac > 1:137092/1‑90 (MQ=255) gagtTGTCCCGCCGGCGGGTCCGGGGGGCCGGAACCCTCAAAACCCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGaa < 1:308146/90‑1 (MQ=255) agaTTTCCCGCCGGCGCGTAGGGGGGGCCGGAACGCAAAAAAACCCAAAACCGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc < 1:251020/90‑1 (MQ=255) agaTTTCCCCCCGGCGCGTACTGGGGGCCGGAACGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:22421/90‑1 (MQ=255) ttGTCCCGCCAGCGCGTTTGGGTGGCCGGAACGCTAAAAACCCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAg < 1:55086/89‑1 (MQ=255) ccgcccgcGGGTGGTGGTGGCGGGACCCCTAAAAAACGCAAAAACGACCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGa < 1:254439/90‑1 (MQ=255) gCCAGCGCGTACTGGTGGCCGGAACGCTAAATAACGCAAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAAc < 1:185448/90‑1 (MQ=255) cccAGCGCGTACGGGGGCCCGAAACCCAAAAAAACACAAAAACGACCCGAATAAAACCGCCACTGACACTGACCATTGAACAGTGTGAAc < 2:411300/89‑1 (MQ=255) aGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg > 2:422822/1‑90 (MQ=255) caaaaaCGCCCCGCATAAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCa < 2:19473/88‑1 (MQ=255) aaaCGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGc < 1:107426/90‑1 (MQ=255) aTCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCG‑‑GCGTAAGGCGCTGGCGGCCATGCGAGTAAg > 1:243978/1‑90 (MQ=255) | AACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTAAG > NZ_CP009273/3214044‑3214216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |