| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,710,374 | 0 | T | G | 66.7% | 2.3 / 16.0 | 18 | G156G (GGT→GGG) | yiaD | OmpA family lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (12/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATC > NZ_CP009273/3710285‑3710437 | ggcgCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGggt < 1:171243/90‑1 (MQ=255) aaCACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCAc < 1:399408/90‑1 (MQ=255) cTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGGTTGGTTTTACCCACAACACGGGGGGGGACGAcccg > 2:101601/1‑88 (MQ=255) cTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTAAAGGGGTTGGTTATACCCACAACACGGGGGGGCACGACCTg > 2:125902/1‑90 (MQ=255) gACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGGTAACGGGATTGGTTATACCAACAGCACGGGGGGGGCCGACCCGaa > 2:403092/1‑90 (MQ=255) cGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGGTTGGTTATACCCACAGCACGGGGGGGCACGACCCGAACATGCg > 2:205625/1‑90 (MQ=255) cAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGtctct < 2:149806/90‑1 (MQ=255) ggTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGGCACGACCTGAACATGCGTCTCTCCCa > 1:169680/1‑90 (MQ=255) aCTGAAAGAGTATCCGAAAACGGCGGTTAAAGGGATTGGGTATACCGACAGCACGGGGGGGCACGACCTGAACAAGCGTCTCTCCCCGCa > 1:453667/1‑90 (MQ=255) tGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAAc < 1:125902/90‑1 (MQ=255) agTATCCGAAAACGGCGGTTAACGTGATTGGGTTTACCGACAGCACGGGGGGGCACGACCCGAACAAGCGTCTCTCCCCGCAACGTGCgg > 2:359467/1‑90 (MQ=255) agTATCCGAAAACGGCGGTTAAAGGGATTGGTTATACCGACAACACCGGGGGGCAAGACCCGAAAAAGCGGCTCTCCCCGCAACGCGCgg > 2:364854/1‑90 (MQ=255) aTCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGGGGGCACGACCTGAACATGCGTCTCTCCCCGCAACGTGCGGAtt > 1:239335/1‑90 (MQ=255) aTCCGAAAACGGCGGTTAACGTGATTGGGTTTACCCACAGCACGGGGGGGCACGACCCGAAAAAGCGGCTCTCCCCGCCACGTGCGGGtt > 2:138208/1‑90 (MQ=255) aTCCGAAAACGGCGGTTAACAGGGTTGGGTATAACAACACCACGGGGGGGGACGGCCCGAAAAGGCGTCTCTCCCAGCAACAAGCGGCtt > 2:201147/1‑90 (MQ=255) aTCCGAAAACGGCGGGTAAAGTGGTTTGTTTTACCGACAACACCGGGGGGCCCGACCCGAACAAGCGGCTCTCCCCGCAACGGGCGGGtt > 2:426024/1‑90 (MQ=255) gTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGcgcg < 1:206033/90‑1 (MQ=255) aCGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGa < 1:368092/90‑1 (MQ=255) gTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATc < 1:452757/90‑1 (MQ=255) | GGCGCTAACACCCTGACCGGCGTGGCAATGGTACTGAAAGAGTATCCGAAAACGGCGGTTAACGTGATTGGTTATACCGACAGCACGGGTGGTCACGACCTGAACATGCGTCTCTCCCAGCAACGTGCGGATTCCGTTGCCAGCGCGTTGATC > NZ_CP009273/3710285‑3710437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |