| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,750,765 | 0 | A | C | 56.2% | 13.2 / 16.5 | 16 | G141G (GGT→GGG) | yiaY | L‑threonine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTGCAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCG > NZ_CP009273/3750679‑3750846 | ggAGTAACATTTTTATCAACAATCGCCTTTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCTTTTCAGAGGCCGTAccc < 1:197990/90‑1 (MQ=255) aGTGACATTTTTATAAAAAATCGCCTTTTTGATATGCCGCGCTTCGTCGGTGATGATGCAGAAAGGGGTCATTTCAGGGGCCGTCCccgc < 2:274088/90‑1 (MQ=255) gACATTTTTACAAAAAATCGCCTTTTTGATAGGCCGCGTTTCGTCAGTGATGATGAAAAAAGGGGTCTTTTCAGAGGCCGTCCCCGCCGt < 2:84197/90‑1 (MQ=255) tttATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTAtt > 1:261379/1‑90 (MQ=255) tCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGGCATTTCAGAGGCCGTACCCGCCGGGGTATTGATg > 2:466685/1‑90 (MQ=255) aaTCGCTTTTTTAATATGCCGCGTTTGGTAAGTGATGATGAAAAAGGGGGTTTTTTAAGGGGCCGTCCCCGCCGTGGTATTGATGGCGat < 2:51586/90‑1 (MQ=255) aaTCGCCTTTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTCCCCGCCGTGGTATTGATGGCGat < 1:3473/90‑1 (MQ=255) aTCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGatc > 2:480474/1‑90 (MQ=255) ttttGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCa > 1:73841/1‑90 (MQ=255) atatGCCGCGCTTCGTCAGTGATGATGAAAAAACGGTTCTTTTCAGAGGCCGTCCCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGc < 1:470385/90‑1 (MQ=255) atGCCGCGCTTCGTCGGTGATGAGGAAGAAAGGGGTCTTTTCAGAGGCCGTCCCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCgg < 2:58607/90‑1 (MQ=255) atGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCgg > 1:82440/1‑90 (MQ=255) tCGTCAGTGATGAGGAAGAACCGGGTCATTTCAGAGGCCGTCCCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTGCagag < 2:57845/90‑1 (MQ=255) cataaGTGATGATGAAAAAGGGGGTCATTTCAGGGGCCTCCCCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTGCAGAGc < 1:341027/86‑1 (MQ=255) cGTCAGTGATGAGGAAGAAGCGGGTCTTTTGAGGGGCCGTCCCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTGCAGAGc < 2:237777/90‑1 (MQ=255) gagGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTGCAGCGCGGTCAACGGCCTCGAAATCGGGGATATcgccg > 1:345317/1‑90 (MQ=255) | GGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTGCAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCG > NZ_CP009273/3750679‑3750846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |