| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,781,385 | 0 | T | G | 64.7% | 5.1 / 14.1 | 17 | G396G (GGT→GGG) | envC | murein hydrolase activator EnvC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (11/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.70e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGAT > NZ_CP009273/3781300‑3781455 | ataATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGggcggc > 1:144202/1‑90 (MQ=255) taATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGcc > 1:211756/1‑90 (MQ=255) gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGGCTTCAct > 1:376396/1‑90 (MQ=255) gagCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCCGGGGCGGCCCTcccc > 2:382497/1‑87 (MQ=255) gcgcACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGGGGCCTTCActct > 1:254918/1‑90 (MQ=255) gTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGCCTTCACTCTATTTCTaa > 2:241037/1‑90 (MQ=255) ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGGCCTCCCTCTATTTCGGAATTcgccgc > 2:282439/1‑90 (MQ=255) ggTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGGCAGGGGCGGGCTTCACTCTATTTCGAAATTctcccc > 2:335074/1‑88 (MQ=255) gCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGGCTTCACTCTATTTTGAAATTCGCCCCCAggg > 2:34073/1‑90 (MQ=255) gCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCCCTCTATTTTGAAAATTGCCCCCCggg > 2:157447/1‑90 (MQ=255) gCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGCCTTCACTCTATTTTGAAAATCGCCCCCCggg > 1:461077/1‑90 (MQ=255) cgcgcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCg < 1:373427/90‑1 (MQ=255) cgcgcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCg < 1:386802/90‑1 (MQ=255) gcgGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTc < 1:34073/90‑1 (MQ=255) aGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCcac < 1:282439/90‑1 (MQ=255) ttGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGGCAGGCGGTCAATCCACAGCCgg > 1:93868/1‑89 (MQ=255) cTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTg < 1:157447/90‑1 (MQ=255) gcagcaGTGGCGGTCAGGGGCGGCCTTCACTCTATTTCGAAATTCGCCGCCCGGGGCAGGCGGGCAATCCACAGCCGCGGGTGGGGAGAt > 2:480006/1‑90 (MQ=255) | ATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCCGCCAGGGTCAGGCGGTCAATCCACAGCCGTGGTTGGGAAGAT > NZ_CP009273/3781300‑3781455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |