| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 371,042 | 0 | A | C | 54.5% | 2.7 / 12.9 | 11 | Q43P (CAG→CCG) | mhpT | 3‑(3‑hydroxy‑phenyl)propionate transporter |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base C (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTT > NZ_CP009273/370953‑371117 | gATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCa < 1:164486/90‑1 (MQ=255) aTGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAg < 2:244781/90‑1 (MQ=255) gACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCctt > 1:215072/1‑90 (MQ=255) tCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCAc < 1:465964/90‑1 (MQ=255) tCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCAc < 2:187740/90‑1 (MQ=255) ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCTTTTTCACCCCATaa > 1:249635/1‑90 (MQ=255) ttGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGGATCGCCCCGGCGTTTGCCCTCGGTaa > 2:72016/1‑90 (MQ=255) gTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGGGGCATCGCCCCGGCTTTCGCACTCGATaaaa > 1:56567/1‑90 (MQ=255) gAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATAttt < 2:71043/90‑1 (MQ=255) cAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTc < 1:391919/90‑1 (MQ=255) gcTGGCATTGCGGCGGGTGGCATCGCCCCGGCTTTTGCACTCCATAAAATTCAAATGGGGGGGGTATTTTGGGCCGGGATACTCGGGTTg > 2:149489/1‑90 (MQ=255) ggggggCATCGCCCCGGCTTTCGCACTCGATAAAAAGCAAATGGGCTGGGTATTTAGCGCCGGAATACTCGGGTTGCTACCCGGCGCgtt > 2:190538/1‑90 (MQ=255) | GATGCTGACCATCGGGCTTTGTTTTTTGGTCGCTCTGATGGAAGGGCTGGATCTTCAGGCGGCTGGCATTGCGGCGGGTGGCATCGCCCAGGCTTTCGCACTCGATAAAATGCAAATGGGCTGGATATTTAGCGCCGGAATACTCGGTTTGCTACCCGGCGCGTT > NZ_CP009273/370953‑371117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |