Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273383,7920AC54.5% 6.7 / 10.8 11N181T (AAC→ACC) tauDtaurine dioxygenase
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (0/6);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATG  >  NZ_CP009273/383714‑383860
                                                                              |                                                                    
tCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGc                                                           >  2:31352/1‑90 (MQ=255)
  cGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGCTa                                                         >  2:425731/1‑90 (MQ=255)
          gtccccGGAATAAAAATCCCGCAAACCCGAGGAGGAACATCAACGCGGGCGCGAGGCGGTCGCAAAAACCCCGCCGTTGCTACATCCggt                                                 <  2:32872/87‑1 (MQ=255)
                   aTACAAATCCCGCAAACCCGAGGAGGAACATCAACGCTGGCGGGGGGCGGTCGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAAc                                        <  1:325198/90‑1 (MQ=255)
                     aCAAATCCCGCAAAACCGAGGAGGAACATCAACGCGGGCGCGAGGCGGTCGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGc                                      <  2:188654/90‑1 (MQ=255)
                                aaacCCGAGGAGGAACATCAGCGCGGGGGGGGGGCGGTCGCAAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGc                           <  2:235013/90‑1 (MQ=255)
                                aaacCCGAGGAGGAACATCAACGCGGGCGCGGGGCGGTCGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGc                           <  1:310853/90‑1 (MQ=255)
                                aaaaCCGAGGAGGAACATCAACGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGc                           <  1:31352/90‑1 (MQ=255)
                                           ggaACATCAACGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGgc                >  2:376389/1‑90 (MQ=255)
                                                   aaCGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTtg        >  2:438720/1‑90 (MQ=255)
                                                         ggCGGGAGGCGGTCGCGAAAACCCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGtgaatg  <  2:277342/90‑1 (MQ=255)
                                                                              |                                                                    
TCCGTAAATCGTTCCCGGAATACAAATACCGCAAAACCGAGGAGGAACATCAACGCTGGCGCGAGGCGGTCGCGAAAAACCCGCCGTTGCTACATCCGGTGGTGCGAACGCATCCGGTGAGCGGTAAACAGGCGCTGTTTGTGAATG  >  NZ_CP009273/383714‑383860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑