| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,252,901 | A→C | intergenic (‑126/+297) | psuK ← / ← fruA | pseudouridine kinase/PTS fructose transporter subunit IIBC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,252,901 | 0 | A | C | 94.1% | 59.1 / ‑3.0 | 17 | intergenic (‑126/+297) | psuK/fruA | pseudouridine kinase/PTS fructose transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (13/3); total (14/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01 | |||||||||||
ACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATT > NZ_CP009273/2252816‑2252970 | acaAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAAtt > 2:317835/1‑90 (MQ=255) gATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCagaga > 1:124729/1‑90 (MQ=255) tACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAgg > 2:303575/1‑90 (MQ=255) aCTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGc > 1:415426/1‑90 (MQ=255) aCTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGc > 1:422168/1‑90 (MQ=255) aaGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATg > 1:149964/1‑90 (MQ=255) cATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGc < 2:202100/90‑1 (MQ=255) gtgtTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAgg > 2:438070/1‑90 (MQ=255) gtgtTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAgg > 2:393674/1‑90 (MQ=255) tgtTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGc < 2:28688/90‑1 (MQ=255) tatTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACaa > 1:137413/1‑90 (MQ=255) tgtgCTCATAGTATGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTa > 2:264200/1‑90 (MQ=255) cATAGTATGCATGTTTTACGTAAAGTCAAATACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGcc > 2:305801/1‑90 (MQ=255) aTGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGGGCAGGCTCACAAAATGCGATTAAATGCCTGAAtt > 2:225782/1‑90 (MQ=255) aTGCATGTTTTACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAAtt > 1:261719/1‑90 (MQ=255) tACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGc < 2:111184/48‑1 (MQ=255) tACGTAAAGTCAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGc > 1:111184/1‑48 (MQ=255) | ACAAGAATATAGATACTGCAAAGACGGTTTAAACATGTGTTCATATTTAAACATGTGCTCATAGTATGCATGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATT > NZ_CP009273/2252816‑2252970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |