Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,982,682	(A)8→7	coding (20/483 nt)	BW25113_RS14815 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,982,675	0	A	.	100.0%	51.8 / NA	13	coding (13/483 nt)	BW25113_RS14815	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (7/6);  total (7/6)

ATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTATAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACT  >  NZ_CP009273/2982595‑2982743                                                                                 |                                                                     aTGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTATaaaaaaactt                                                             >  2:520858/1‑87 (MQ=255)    cAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACttatt                                                         >  2:192661/1‑90 (MQ=255)              tataACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTAttttg                                               >  1:333240/1‑89 (MQ=255)                          gAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGt                                   <  2:433390/90‑1 (MQ=255)                               ggCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGtt                              <  1:403889/90‑1 (MQ=255)                                 cAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTa                            >  2:384513/1‑90 (MQ=255)                                  aaaCTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTGGTTTTTATCAGTGGGTTTAt                           <  2:333240/90‑1 (MQ=255)                                              ccTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGt               <  1:381769/90‑1 (MQ=255)                                               cTGTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTc              >  2:16350/1‑90 (MQ=255)                                                 gTCTTACCGTGAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTa            >  1:135076/1‑90 (MQ=255)                                                           gAGGTTCATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACt                 <  1:306780/75‑1 (MQ=255)                                                           gAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACt                 >  2:306780/1‑75 (MQ=255)                                                           gAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACt  >  1:246510/1‑90 (MQ=255)                                                           gAGGTTAATCGTGATTGATTAT‑AAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACt  <  2:259310/90‑1 (MQ=255)                                                                                 |                                                                     ATGCAATAAAAACTATAACCAACATGAAGTGGCAAACTGTTTAACCCTGTCTTACCGTGAGGTTAATCGTGATTGATTATAAAAAAAACTTATTATTTATTTTAGTTTTTATCAGTGGGTTTATTCTCTTCACTGTCTATTCTTACACT  >  NZ_CP009273/2982595‑2982743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑