| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,299,745 | (A)6→5 | coding (1053/1533 nt) | alsA ← | D‑allose ABC transporter ATP‑binding protein AlsA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,299,740 | 0 | A | . | 95.0% | 72.3 / ‑1.1 | 20 | coding (1058/1533 nt) | alsA | D‑allose ABC transporter ATP‑binding protein AlsA |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (6/13); total (6/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
ATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTT > NZ_CP009273/4299668‑4299825 | aTCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCcggc < 2:92043/90‑1 (MQ=255) ccTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCttt > 2:251637/1‑90 (MQ=255) ttATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAGAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAg < 2:79015/90‑1 (MQ=255) tataGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGt < 1:251637/90‑1 (MQ=255) cgccgTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTa > 2:467429/1‑90 (MQ=255) gCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTc > 1:386712/1‑90 (MQ=255) gCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTc > 2:62170/1‑90 (MQ=255) gCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTc > 2:322945/1‑90 (MQ=255) cTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACgg < 2:328514/90‑1 (MQ=255) cTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACgg < 2:371345/90‑1 (MQ=255) ccATGTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAg < 2:414185/90‑1 (MQ=255) gTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGt < 1:351674/90‑1 (MQ=255) gTTCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGt < 1:62170/90‑1 (MQ=255) ttCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTCATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGt < 1:281557/90‑1 (MQ=255) tCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGa < 1:104385/90‑1 (MQ=255) tCTGAGCGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGa < 2:485784/90‑1 (MQ=255) cGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTgg < 2:32960/90‑1 (MQ=255) cGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTgg < 1:467429/90‑1 (MQ=255) cGATGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTgg < 1:228585/90‑1 (MQ=255) aTGGAAAAGTTGGGG‑AAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGgag > 2:224391/1‑90 (MQ=255) tggggAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCttt > 1:333288/2‑90 (MQ=255) | ATCGCGCCTTTATAGCCGCCGTCTTTCAGACTGCGGCTGATCGCCATGTTCTGAGCGATGGAAAAGTTGGGGAAAAAACCGTTATCCCGGCGGCTTTCAGTGATGTAAGCCATCCCTTTTTTCACGGCATCCAGGGGTGAACGTGGAGAGATATCTTT > NZ_CP009273/4299668‑4299825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |