| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 982,464 | (T)8→7 | intergenic (‑26/+577) | ompF ← / ← asnS | porin OmpF/asparagine‑‑tRNA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 982,457 | 0 | T | . | 88.9% | 25.5 / ‑2.7 | 9 | intergenic (‑19/+584) | ompF/asnS | porin OmpF/asparagine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (3/5); total (3/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTAT > NZ_CP009273/982396‑982542 | aCAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCa > 2:156081/1‑90 (MQ=255) gagCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATa > 2:66904/1‑90 (MQ=255) aaTATTGCGCTTCATCATTATTTATTACCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTAc < 1:82328/90‑1 (MQ=255) atTGCGCTTCATCATTATTTATTCCCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGa < 1:502022/90‑1 (MQ=255) tGCGCTTCATCATTATTTATTCCCCTCATGG‑TTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAAc < 1:432514/90‑1 (MQ=255) tattaCCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTgg < 1:214526/90‑1 (MQ=255) ttccccTCAT‑GTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgtg < 2:201980/87‑1 (MQ=255) ttaCCCTCATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGgtg < 1:113004/90‑1 (MQ=255) cATGG‑TTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTAt > 2:332648/1‑90 (MQ=255) | ACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTAT > NZ_CP009273/982396‑982542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |