Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,095,349	Δ1 bp	coding (254/555 nt)	ycdY →	molecular chaperone YcdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,095,347	0	G	.	100.0%	46.8 / NA	12	coding (252/555 nt)	ycdY	molecular chaperone YcdY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (10/2);  total (10/2)

AGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCG  >  NZ_CP009273/1095272‑1095426                                                                            |                                                                                aGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGA‑GGCGCGAcggaagcg                                                                  >  1:482391/1‑90 (MQ=255)      ctGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGA‑GGCGCGACGGAAGCGGAAGt                                                             >  2:79344/1‑90 (MQ=255)                  aTGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTtttct                                                 >  2:279028/1‑90 (MQ=255)                      gttgttTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTttctttct                                             >  2:387646/1‑90 (MQ=255)                       ttgttTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTg                                            >  2:436893/1‑90 (MQ=255)                         gttTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAg                                          >  2:330684/1‑90 (MQ=255)                             aTCGGGGAGGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAg                                      <  2:9957/90‑1 (MQ=255)                                                    gccATATCGTAGCGCATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCa               >  1:130358/1‑90 (MQ=255)                                                           cGTAGCGCATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATc        <  1:330684/90‑1 (MQ=255)                                                                 gcATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCg  >  1:118127/1‑90 (MQ=255)                                                                 gcATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCg  >  1:224874/1‑90 (MQ=255)                                                                 gcATGGGTTGA‑GGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCg  >  2:265498/1‑90 (MQ=255)                                                                            |                                                                                AGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCG  >  NZ_CP009273/1095272‑1095426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑