BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,615,927	(T)_5→4	coding (339/1188 nt)	ydeE →	efflux MFS transporter YdeE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,615,923	0	T	.	100.0%	69.7 / NA	17	coding (335/1188 nt)	ydeE	efflux MFS transporter YdeE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (6/11); total (6/11)

TACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTACAC  >  NZ_CP009273/1615858‑1616004

tACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTgg                                                          >  1:290612/1‑90 (MQ=255)

           aTAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACa                                                <  1:287899/89‑1 (MQ=255)

           aTAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACa                                                >  2:287899/1‑89 (MQ=255)

           aTAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG‑TTTTGCTACCATGCTGAAAGcc                                                             >  2:89022/1‑76 (MQ=255)

           aTAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG‑TTTTGCTACCATGCTGAAAGcc                                                             <  1:89022/76‑1 (MQ=255)

            tAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG‑TTTTGCTACCg                                                                        >  2:89445/1‑64 (MQ=255)

            tAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG‑TTTTGCTACCg                                                                        <  1:89445/64‑1 (MQ=255)

                           tgtgCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGc                               <  2:425921/90‑1 (MQ=255)

                                              aTTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCtcaa            <  1:481771/90‑1 (MQ=255)

                                              aTTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCtcaa            <  1:325260/90‑1 (MQ=255)

                                              aTTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCtcaa            <  2:290612/90‑1 (MQ=255)

                                              aTTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCtcaa            <  2:302572/90‑1 (MQ=255)

                                              aTTAACTGCGCCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCtcaa            <  2:66852/90‑1 (MQ=255)

                                                       gcCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAAc       >  2:305669/1‑86 (MQ=255)

                                                       gcCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAAc       <  1:305669/86‑1 (MQ=255)

                                                       gcCTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTaca   >  1:90511/1‑90 (MQ=255)

                                                        ccTATTCTG‑TTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTacac  <  1:325200/90‑1 (MQ=255)

TACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTGTTTTTGCTACCGTGCTGAAAGCCTGGTTTGCCGACAATCTTTCGTCCACCAGCAAAACGAAAATCTTCTCAATCAACTACAC  >  NZ_CP009273/1615858‑1616004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`