| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,769,167 | (A)5→4 | coding (225/759 nt) | aroD → | type I 3‑dehydroquinate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,769,163 | 0 | A | . | 95.5% | 85.7 / ‑1.4 | 22 | coding (221/759 nt) | aroD | type I 3‑dehydroquinate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (12/9); minor base G (0/1); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATAT > NZ_CP009273/1769082‑1769235 | ggCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCgct > 2:105614/1‑90 (MQ=255) tgGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGtt < 2:207692/90‑1 (MQ=255) tGCCGACCTCTCCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGc < 2:59515/90‑1 (MQ=255) ccGACCTCTCCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAg < 2:224839/90‑1 (MQ=255) gACCTCTCCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTg > 2:153971/1‑90 (MQ=255) ctcCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGc > 1:147326/1‑81 (MQ=255) ctcCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGc < 2:147326/81‑1 (MQ=255) ctcCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCaaa > 1:479354/1‑90 (MQ=255) gAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGgcggcg > 1:513723/1‑90 (MQ=255) gAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGgcggcg > 1:91780/1‑90 (MQ=255) gAGTCTGTCATAGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTG‑TTACCTTCCGCAGTGCCAAAGAAGGCGGCGa > 1:471557/1‑90 (MQ=255) cTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCa > 1:89068/1‑90 (MQ=255) gcggcAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGAttt > 1:366104/1‑90 (MQ=255) gcggcAGCAAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGAttt > 1:136837/1‑90 (MQ=255) cagcaAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCAc > 1:277173/1‑90 (MQ=255) cagcaAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCAc < 1:153971/90‑1 (MQ=255) gcaAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCg < 1:105614/90‑1 (MQ=255) gcaAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCg < 2:136837/90‑1 (MQ=255) gcaAAAATTCTCCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCg < 2:366104/90‑1 (MQ=255) gcaAAAATTCTCCGTGAGACCATGCC‑GGAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCg < 1:59382/90‑1 (MQ=255) tcCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTatat < 1:71514/90‑1 (MQ=255) tcCGTGAGACCATGCCAG‑AAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTatat > 2:50453/1‑90 (MQ=255) | GGCGTGTGGACCACTATGCCGACCTCTCCAATGTGGAGTCTGTCATGGCGGCAGCAAAAATTCTCCGTGAGACCATGCCAGAAAAACCGCTGCTGTTTACCTTCCGCAGTGCCAAAGAAGGCGGCGAGCAGGCGATTTCCACCGAGGCTTATAT > NZ_CP009273/1769082‑1769235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |