Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	375,377	(G)6→7	intergenic (‑40/+148)	frmR ← / ← yaiO	formaldehyde‑responsive transcriptional repressor FrmR/YaiO family outer membrane beta‑barrel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	375,371	1	.	G	100.0%	25.8 / NA	9	intergenic (‑34/+154)	frmR/yaiO	formaldehyde‑responsive transcriptional repressor FrmR/YaiO family outer membrane beta‑barrel protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (3/6);  total (3/6)

GAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑GGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGG  >  NZ_CP009273/375283‑375440                                                                                          |                                                                      gAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑g                                                                      >  1:407474/1‑90 (MQ=255)       gacgaACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑ggggggg                                                                <  2:155979/90‑2 (MQ=255)                gAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGGTATTCtata                                                        <  2:407474/90‑1 (MQ=255)                                cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGGTATTCTATATGTCAATGCATAcccc                                        <  1:332967/90‑1 (MQ=255)                                     cttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGGTATTCTATATGTCAATGCATACCCCCCtat                                   <  1:437271/90‑1 (MQ=255)                                               aCTGGGCATTTCGCCCCTCATCATCTGCATGCAATATACTATAGGGGGGGTATTCTATATGTCAATGCAt                                             <  2:194124/70‑1 (MQ=255)                                               aCTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGGTATTCTATATGTCAATGCAt                                             >  1:194124/1‑70 (MQ=255)                                                  gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGGTATTCTATATGTCAATGCATACCCCCCtat                                   >  1:302771/1‑77 (MQ=255)                                                  gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGGTATTCTATATGTCAATGCATACCCCCCtat                                   <  2:302771/77‑1 (MQ=255)                                                  gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACt                      >  1:29235/1‑90 (MQ=255)                                                                      tcTGCATGCAATATACTATAGGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCgg  <  1:427767/90‑1 (MQ=255)                                                                                          |                                                                      GAATACGACGAACTCGAGTAAGGACCTTTTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATA‑GGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGG  >  NZ_CP009273/375283‑375440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑