| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,596,032 | (G)8→9 | noncoding (72/87 nt) | BW25113_RS22750 ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,596,024 | 1 | . | G | 92.9% | 41.1 / ‑2.1 | 14 | noncoding (80/87 nt) | BW25113_RS22750 | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (5/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01 | |||||||||||
ACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC > NZ_CP009273/4595936‑4596094 | acacTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑g < 2:252074/90‑1 (MQ=255) cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg < 2:509411/90‑1 (MQ=255) tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggg‑ggggggact > 1:336866/1‑86 (MQ=255) tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggg‑ggggggact > 1:40647/1‑86 (MQ=255) gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggg‑ggggg < 2:94185/74‑1 (MQ=255) gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑ggg‑ggggg > 1:94185/1‑74 (MQ=255) ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGa < 2:398763/90‑1 (MQ=255) gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGG‑GGGGGACTTGAACCCCCAc > 2:42751/1‑77 (MQ=38) gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGG‑GGGGGACTTGAACCCCCAc < 1:42751/77‑1 (MQ=38) gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTaa < 2:260389/84‑1 (MQ=255) gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTaa > 1:260389/1‑84 (MQ=255) cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTa < 2:40647/90‑1 (MQ=255) ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAac < 2:310142/90‑1 (MQ=255) ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAacac > 2:360499/1‑90 (MQ=255) cGCACAGTCATCTTACTTTTTTTGAAATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCt < 2:268577/90‑1 (MQ=25) gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt > 1:103992/1‑90 (MQ=255) gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGTGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt > 1:41389/1‑90 (MQ=255) ttttGATATCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa > 2:504390/1‑90 (MQ=255) atCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:103992/90‑1 (MQ=255) atCGCCTCGTTTGGTGCGAG‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 1:360499/90‑1 (MQ=255) | ACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC > NZ_CP009273/4595936‑4596094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |