| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 261,760 | 0 | A | C | 53.8% | 9.2 / 12.0 | 13 | G13G (GGT→GGG) | BW25113_RS01265 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAG > NZ_CP009273/261672‑261845 | cGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTacac > 1:261007/1‑90 (MQ=255) cACGCTGTATCCCGTATTGCCCTGCCCGTACCCCTGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGc < 1:379679/90‑1 (MQ=255) aCGCTGTATTCCGTATTGCCCTGACCGTACCCCTGCTGCCAGACCCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACCCCCGcc < 2:102373/90‑1 (MQ=25) tATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATa > 1:128067/1‑90 (MQ=255) aCCGTAACCCTGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACCCCCGCCAGCATAAACCGACTTAGTGAAAg < 1:450335/90‑1 (MQ=255) ccGTACCCCTGCTGCCAGACCCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACCCCCGCCAGCATAAACCGACTTAGTGAAAGt < 1:334894/90‑1 (MQ=255) ccGTACCCCTGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACCCCCGCCAGCATAAACCGACTTAGTGAAAGt < 1:336798/90‑1 (MQ=255) ctgctgCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCAt > 2:117122/1‑90 (MQ=255) caaaCCCCGGGAATAGCAGAGGCATTAAAGCCCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGtc < 1:380413/88‑1 (MQ=255) aGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGtct > 2:385440/1‑90 (MQ=255) gaaaGCAGAGGAATTAAAGCTCGCGAGCATTCCCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCgt < 2:233957/87‑1 (MQ=255) gttACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGa < 2:69462/88‑1 (MQ=255) aGTACCCCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGa < 1:391970/90‑1 (MQ=255) gTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAg > 1:77150/1‑90 (MQ=255) | CGGTCACGCTGTATTCCGTATTGCCCTGACCGTAACCCTGCTGCCAGAACCCCGGG‑ATAGCAGAGGCATTAAAGCTCGCGAGCAGTACACCCGCCAGCATAAACCGACTTAGTGAAAGTATTGTCATTCTTGTCTCCTTTGTCGTTGTTTTATTCCTGATTGTCAGGGTTCGAG > NZ_CP009273/261672‑261845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |