| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 443,152 | 0 | T | A | 58.3% | 0.2 / 10.9 | 12 | K4* (AAG→TAG) | cyoE | heme o synthase |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base A (0/7); total (3/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATGGAGCCTA > NZ_CP009273/443065‑443225 | tgcCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTaa < 2:104555/90‑1 (MQ=255) gcCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTaaa > 2:351633/1‑90 (MQ=255) agcagGAACCCCCCAACCCCCGAGACCGGTTTCCAAAAGATGATGCCTGTTTTCTTTACTTGCGGTTATTGTTAAAACATCAAACccgcc < 2:412859/90‑1 (MQ=255) agcagAAACCCCCCAACCCCCGAGATCGGTTTCCAAAAGATGATGCCTGTTTTCTTTACTTGCGGGTATTGTTAAAAAATCATAAccgcc < 2:398964/90‑1 (MQ=255) aaccccccACCCCCCGAGATCAGTTTCCAAAAGATGATGCCTGTTTTCTTTCCTTGCAGGTTTTGCTAAAACATCAAAACCGCCGCTCtt < 2:3541/87‑1 (MQ=255) ccACCGGAATCGGGTTGCAAAAGATGCGGCCTGGTTTTGTTCCTTGCAGATATTGCTTAAACAACATAACCGCCGCTCTTAGTGcatcat < 2:362082/89‑1 (MQ=255) agaTCGGTTTCCAAAAGATGTTGCTTGTTTTCTTTTTTTGGGGTTTTTGTTAAAACATCAAAACCGCCGCTCTTAGTGCATCATCAtgtt < 2:221296/90‑1 (MQ=255) accAGGTTACAAAAGATGATCCTTTTTTTCTTTCCTTGCAGTTATTGCTAAAACATCATAACCGCCGCTCTTAGTGCATCATCAtgttgt < 2:223735/88‑1 (MQ=255) aGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCca > 1:12327/1‑90 (MQ=255) ggTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGa > 1:203176/1‑90 (MQ=255) tctttACTTGCGGTTTTTGTTAAAACATCAAACCCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATGGa < 2:427009/87‑1 (MQ=255) aCTTGCAGTTTTTGTTAAAACATCAAACCCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATGGAGCCTa < 2:196058/90‑1 (MQ=255) | TGCCCTTTGAGGCCAGCAGGAATCCCCCAATCACCGAGATCAGGTTGCCAAAGATGATGCCTGGTTTCGTTACTTGCAGGTATTGCTTAAACATCATAACCGCCGCTCTTAGTGCATCATCATGTTGTAGTTGAGGTTCCACATAATCCAGATGGAGCCTA > NZ_CP009273/443065‑443225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |