| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 536,014 | 0 | T | G | 61.5% | 5.4 / 11.9 | 13 | intergenic (+50/‑7) | allB/ybbY | allantoinase AllB/uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (8/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGT > NZ_CP009273/535930‑536100 | cGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGtt < 2:163094/90‑1 (MQ=255) aaaCATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTATGTTCAATTTTGCAGTCa > 1:187420/1‑90 (MQ=255) tCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTATGTTTAATTTTGCAGTCAGCcgc > 1:140550/1‑90 (MQ=255) cagTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGaaa < 2:45707/90‑1 (MQ=255) aaTCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGGATTCTCCGGTTAAGGGGGGTTTTTTTTTAATTTTTCAGTCAGCCGCGAAAGCCt > 2:111100/1‑90 (MQ=255) ggCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGGGTGTTTTTGTTTAATTTTTCAGTCAGCCGCGAAAGCCTGtttt > 1:296280/1‑88 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTTTCCGGTTAAGGGGGGGTTTTGTTTAATTTTTCAGTCAGCCGCGAAAGCCTGTTTTCAggg > 1:293613/1‑89 (MQ=255) cTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGGTAAGGGGGGTTTTTGTTTAATTTTGCAGTCAGCCGCGAAAACCTGTTTTTAGGa > 1:232922/1‑90 (MQ=255) aTGCCCGTCCTTGCGGCGGGGATTTTCCGGGTAAGGGGGGGTTTTTTTTAATTTTGCAGTCAGCCGCGGAAGGCCGTTTTTAGGGtttta > 2:311998/1‑88 (MQ=255) gggCATTCTCCGGTTAAGGGGGGTTTTTGTTCAATTTTTCAGTCAGCCGCGAAAGCCTGTTTTCAGGGTTTTAGTGGTtttttttttttt > 2:487548/1‑90 (MQ=255) tcCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTACTTTTTGCAac < 2:462203/90‑1 (MQ=255) cGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAacac < 2:504715/90‑1 (MQ=255) aGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTtgt > 2:25613/1‑90 (MQ=255) | CGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCCCTGCAATGCCCGTCCTTGCGGCGGGCATTCTCCGGTTAAGGTGTGTTTATGTTCAATTTTGCAGTCAGCCGCGAAAGCCTGTTATCAGGATTTCAGTGGTTTTTCTTTATTTTTTGCAACACGGTTGT > NZ_CP009273/535930‑536100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |