Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273832,0480TG53.8% 10.9 / 16.9 13V81G (GTC→GGC) hcxBhydroxycarboxylate dehydrogenase HcXB
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (7/0);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03
Rejected as consensus: Frequency below/above cutoff threshold.

GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGG  >  NZ_CP009273/831962‑832107
                                                                                      |                                                           
gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCAc                                                          >  1:448953/1‑90 (MQ=255)
           aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGTCGGGGGCGGCGGTCACGCTCGATGGCg                                               >  1:97127/1‑90 (MQ=255)
           aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCCCGAAGGCg                                               >  2:422335/1‑90 (MQ=255)
                cGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATcgc                                          <  1:422335/90‑1 (MQ=255)
                  cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGGCACCCCCCATGGCGGTCgcgc                                        >  2:183532/1‑90 (MQ=255)
                            cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCCCGATGGGGGTCGCGCCTTTTGTCAg                              >  1:432682/1‑90 (MQ=255)
                            cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCCCCAAGGGGGGCGCGCATTTTGGCAg                              >  1:453741/1‑90 (MQ=255)
                            cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATcgc                                          >  1:106854/1‑78 (MQ=255)
                            cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATcgc                                          <  2:106854/78‑1 (MQ=255)
                              ggggCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCTCGATGGCGAACGCGCATTTTGTCAGGt                            >  2:191236/1‑90 (MQ=255)
                                  cACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTcgcg                        <  2:448953/90‑1 (MQ=255)
                                                      tGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCCCGATGGGGATCGCGCCTTTTGTCAGGGCGCGGCACATGAAGCGGTGGGGCt    >  1:502139/1‑90 (MQ=255)
                                                        ccAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTgg  <  1:126604/90‑1 (MQ=255)
                                                                                      |                                                           
GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGG  >  NZ_CP009273/831962‑832107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑