| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,634,789 | 0 | A | C | 56.2% | 10.5 / 16.0 | 16 | G18G (GGT→GGG) | essD | phage lysis protein EssD |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/9); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAAT > NZ_CP009273/1634708‑1634874 | cAGGCTCCCCAGCACCCCGATTGCCCCCCCCTGTGAGGGATTTTTTTTATCGAGTAACTGAAATGCCAAAAACCCAGCATTCCCCGCCGa < 2:310773/90‑1 (MQ=255)cAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGa > 1:225543/1‑90 (MQ=255) cccACCACCCCGTTTGCACCCCCCTGTGACGGATTTATTTTATCGAGTAACTGAAATGCCCAAAACCCAGCTTTCCCCGCCGATGTGCCa < 2:225543/90‑1 (MQ=255) acacCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCa < 1:14803/90‑1 (MQ=255) agttGCAGCCCACTGTGACGGATTTTTTTTATCGAGTAACTGCAATGCCCAAAACCCAGCATTCCCCGCCGATGTGCCATAGGCAACAcc < 1:373456/88‑1 (MQ=255) aTTGCACCCCACTGTGACGGATTTCTTTTATCGAGTAACTGCAATGCCCAAAAACCAGCTTTCCCCGCCGATGTGCCATAGGCAACACCt < 2:485678/90‑1 (MQ=255) ttGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCtg > 1:378307/1‑90 (MQ=255) aGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCtgttgt > 1:485678/1‑90 (MQ=255) cccATTGTGAGGGAGTTACTTTATCGAGTAACTCCAATCCCCAAAACCCACCATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTa < 2:401153/90‑1 (MQ=255) cTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAAcct > 2:373456/1‑88 (MQ=255) tttATCGAGTAACTGCAATGCCCAAAAACCAGCATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCat < 1:207755/90‑1 (MQ=255) cGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCt > 2:508673/1‑90 (MQ=255) ttaaaTGCCCAGAAACCATTTTTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGa < 1:268710/87‑1 (MQ=37) tgaaaTGCCCAAAACCCAGCATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGa < 1:61286/87‑1 (MQ=37) gCCAAGAAACAACCATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTaca < 2:292602/90‑1 (MQ=37) aaaCCAGCATTCCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATg < 1:356862/90‑1 (MQ=255) gctcaCCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAat < 2:272385/86‑1 (MQ=255) | CAGGCTACCCAGCACACCGATTGCAGCCCACTGTGACGGAGTTACTTTATCGAGTAACTGCAATGCCCAGAAACCAGCATTACCCGCCGATGTGCCATAGGCAACACCTGTTGTTAACTTATCCATTGATTTCATATCCTCACCCCGATGTACACGGATGGTGCAAT > NZ_CP009273/1634708‑1634874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |