| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,673,945 | 0 | T | G | 56.2% | 4.5 / 18.9 | 16 | G44G (GGT→GGG) | ydgI | amino acid permease |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base G (9/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTA > NZ_CP009273/1673863‑1674030 | cAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGGATTACTg > 1:184499/1‑90 (MQ=255)cAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGGATTACTg > 1:328366/1‑90 (MQ=255)cAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGGATTACTg > 1:479004/1‑90 (MQ=255)cAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGGTGCCAGCCCGGCGGGACTGCTCCTCGGCTGGGGGATTTCtt > 2:320252/1‑89 (MQ=255) aTGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTggg > 2:267180/1‑89 (MQ=255) aTGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGGATTACTGgc > 1:334319/1‑90 (MQ=255) cTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGGATTACTGGCGCt > 1:321263/1‑90 (MQ=255) gggCGCGGGTGTTTTCAGTCTGCCGCAAAATGTGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGGATTACTGGCGCTgg > 1:397807/1‑90 (MQ=255) gggCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCGGGGGGATTAATGGCGCTgg > 1:127624/1‑90 (MQ=255) ggCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGc < 2:321263/90‑1 (MQ=255) tCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCtt < 2:479004/90‑1 (MQ=255) gCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGc < 1:267180/90‑1 (MQ=255) aaaTATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGGATTTCTGGGGCTGGCATTTTTTTTCTGGGCCTTTCCATGCt > 2:254971/1‑90 (MQ=255) aTCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGt < 1:254971/90‑1 (MQ=255) tGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCttt > 1:180167/1‑90 (MQ=255) ggggTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATACTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTa > 1:439256/1‑90 (MQ=255) | CAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTA > NZ_CP009273/1673863‑1674030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |