| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,103,492 | 0 | A | G | 64.3% | 7.1 / 11.5 | 14 | V43A (GTC→GCC) | rfbC | dTDP‑4‑dehydrorhamnose 3,5‑epimerase |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base G (2/7); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTATCTTGTGCGTACTCGCCGCGTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGACCGGATAGCCTAGAATATGTTCAAATGCTGATTGATTAAAGCTCTCATAAAAGAAACCTCTATCATCACCAAATACTCTTGGC > NZ_CP009273/2103405‑2103575 | ttATCTTGTGCGTACTCGCCGCGTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGGcc > 1:338094/1‑90 (MQ=255) cTTGTGCGTACTCGCCGCGTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGGCCGGAt < 2:177210/90‑1 (MQ=255) cTTGTGCGTACTCGCCGCGTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGGCCGGAt < 2:220084/90‑1 (MQ=255) cTTGTGCGTACTCGCCGCGTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGACCGGAt < 1:202332/90‑1 (MQ=255) cTTGTGCGTACTCGCCGCGTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGACCGGAt < 2:463600/90‑1 (MQ=255) gtgCGTACTCGCCGCGTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGACCGGATAGc > 2:109543/1‑90 (MQ=255) gTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGGCCGGATAGCCTAGAATATGTTCaa < 2:338094/90‑1 (MQ=255) tGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGGCCGGATAGCCTAGAATATGTTCAAATGCtgat < 1:440660/90‑1 (MQ=255) cATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGGCCGGATAGCCTAGAATATGTTCAAATGCTGATTGATTAAAGCTCTCAt < 1:134059/90‑1 (MQ=255) tttGATGAACGTGAGTGATTGTCTTGAACAAAGCTGACCGGATAGCCTAGAATATGTTCAAATGCTGATTGATTAAAGct > 1:1050/1‑80 (MQ=255) tttGATGAACGTGAGTGATTGTCTTGAACAAAGCTGACCGGATAGCCTAGAATATGTTCAAATGCTGATTGATTAAAGct < 2:1050/80‑1 (MQ=255) cGTGAGTGATTGTCTTGAACAAAGCTGGCCGGATAGCCTAGAATATGTTCAAATGCTGATTGATTAAAGCTCTCATAAAAGAAACCTCTa < 2:189223/90‑1 (MQ=255) cAAAGCTGGCCGGATAGCCTAGAATATGTTCAAATGCTGATTGATTAAAGCTCTCATAAAAGAAACCTCTATCATCACCAAATACTCTTg < 2:209623/90‑1 (MQ=255) aaGCTGGCCGGATAGCCTAGAATATGTTCAAATGCTGATTGATTAAAGCTCTCATAAAAGAAACCTCTATCATCACCAAATACTCTTGGc > 2:432723/1‑90 (MQ=255) | TTATCTTGTGCGTACTCGCCGCGTTGAAAGTGAAGGCCTCTGAGTACATTTTTTGATGAACGTGAGTGATTGTCTTGAACAAAGCTGACCGGATAGCCTAGAATATGTTCAAATGCTGATTGATTAAAGCTCTCATAAAAGAAACCTCTATCATCACCAAATACTCTTGGC > NZ_CP009273/2103405‑2103575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |